CORE SERVICE FOR FLOW CYTOMETRY

流式细胞术核心服务

• 批准号: 8172956
• 负责人: Marcelo J Kuroda
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172956
• 关键词: Acquired Immunodeficiency Syndrome; Cell Proliferation; Cells; Color; Computer Retrieval of Information on Scientific Projects Database; Data Analyses; Data Files; Esters; Flow Cytometry; Fluorescein; Fluoresceins; Funding; Grant; Individual; Institution; Laboratories; Lasers; Monoclonal Antibodies; Ploidies; Preparation; Primates; Process; Research; Research Personnel; Resources; Sampling; Secure; Services; Side; Sorting - Cell Movement; Source; Speed; United States National Institutes of Health; data acquisition; digital; instrumentation; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Flow Cytometry Core Laboratory instrumentation includes 2 Becton-Dickinson (BD) FACSCaliburs, a BD LSRII and a BD FACSAria. The FACSCaliburs are capable of 6 parameter analysis (4 colors plus forward and side scatter). They are used for 4-color acquisitions, cell proliferation studies using carboxy-fluorescein diacetate succinimidyl ester (CFSE) and cell ploidy determinations. Both the LSRII and the FACSAria are used for multiparameter sample determinations. Each is a digital platform containing 3 lasers and capable of 14 parameter analysis (12 colors plus forward and side scatter). The FACSAria is also a high speed cell sorter, capable of aseptically sorting up to 4 subpopulations simultaneously with exceptional purity. The needs of individual investigators varied. Some investigators utilized the comprehensive services offered by the core lab: from monoclonal antibody selection through data acquisition. The majority of investigators processed their own samples in preparation for flow cytometry acquisition. The core lab provides limited data analysis; most investigators perform their own data analysis. Raw data files are distributed to the various investigators via a secure Tulane National Primate Research Center server. Flow Cytometry Laboratory provided services with 4 types of analyses. Service totals for 2009 are as follows: Calibur = 2840; Multicolor = 14859; Sort = 41 and TruCount = 1820. These services were provided to both core and affiliate investigators. The AIDS research program is the largest user of the Core.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 流式细胞术核心实验室仪器包括 2 个 Becton-Dickinson (BD) FACSCaliburs、一个 BD LSRII 和一个 BD FACSAria。 FACSCaliburs 能够进行 6 种参数分析（4 种颜色加上前向和侧向散射）。 它们用于 4 色采集、使用羧基荧光素二乙酸琥珀酰亚胺酯 (CFSE) 的细胞增殖研究以及细胞倍性测定。 LSRII 和 FACSAria 均用于多参数样品测定。 每个都是一个数字平台，包含 3 个激光器，能够进行 14 种参数分析（12 种颜色加上前向和侧向散射）。 FACSAria 也是一款高速细胞分选仪，能够同时对多达 4 个亚群进行无菌分选，且纯度极高。 个别研究者的需求各不相同。一些研究人员利用了核心实验室提供的综合服务：从单克隆抗体选择到数据采集。大多数研究人员处理自己的样本，为流式细胞术采集做准备。核心实验室提供有限的数据分析；大多数研究人员都会进行自己的数据分析。原始数据文件通过安全的杜兰国家灵长类动物研究中心服务器分发给各个研究人员。 流式细胞术实验室提供4种分析服务。 2009 年服务总数如下： Calibur = 2840；多色 = 14859； Sort = 41，TruCount = 1820。这些服务同时提供给核心研究人员和附属研究人员。艾滋病研究计划是 Core 的最大用户。