A Biacore T200 for Molecular Interaction Studies at the University of Miami

迈阿密大学用于分子相互作用研究的 Biacore T200

• 批准号: 8247257
• 负责人: RALF LANDGRAF
• 金额: $ 35.1万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247257
• 关键词: Antibodies; Applied Research; Basic Science; Binding; Clinical Research; Comprehensive Cancer Center; DNA; Detection; Equipment; Evaluation; Florida; Fostering; Funding; Future; Health; Housing; Human; Institutes; Label; Lead; Maintenance; Measurement; Medical; Methods; Modality; Molecular Weight; Nucleic Acids; Outsourcing; PTPRC gene; Pharmaceutical Preparations; Property; Proteins; Radial; Research; Research Personnel; Sampling; Serum; Structure; Surface Plasmon Resonance; Thermodynamics; United States National Institutes of Health; Universities; aptamer; falls; improved; instrument; instrumentation; medical schools; novel; protein complex; public health relevance; small molecule

DESCRIPTION (provided by applicant): The six basic research departments at the University of Miami, Miller School of Medicine carry out a broad range of basic science studies with strong translational components. Interdepartmental structures like the Comprehensive Cancer Center (which contributes to the maintenance of the proposed SPR equipment) foster interactions between basic researchers and with clinical research groups. Among this combined group of researchers (including one user from the neighboring Florida Scripps Institute), a strong need exists for modalities that allow highly sensitive and label free molecular interaction studies with small sample volumes. Surface Plasmon Resonance has become a major, if not the dominant, method of analysis for this type of applications in the last decade, especially with its increase in sensitivity in recent years (which now includes the label free binding studies of small molecule analytes) and the ability to multiplex and automate measurements. This allows for a broad range of studies to be carried out efficiently and with minimal sample volumes. Our high demand for SPR capability is in stark contrast to the absence of any such equipment with open access within a several hundred mile radius of UM. The absence of SPR capability forces researchers to rely on less sensitive and less informative methods at best. In some cases, such label free binding of novel, small molecule lead compounds, no other options are available. In yet other cases (antibody optimization) outsourcing is currently the only option The proposed purchase of a Biacore T200 would establish much needed SPR capability on the UM campus. The instrument will be housed in a supervised instrumentation facility will be maintained through institutional support. Featured applications include the characterization and optimization of therapeutically used antibodies, the evaluation of existing or de novo synthesized small molecular weight drugs, the detailed comparison of thermodynamic binding properties between ITC and SPR as alternative methods, the assembly of multi protein complexes on DNA, the characterization of SELEX derived nucleic acid aptamers to target proteins, and the detection of analyte concentrations in serum samples, and several projects emphasizing the strength of SPR in the study of weak interactions All applications fall within the capability range of a Biacore T200, and availability of SPR is certain to enhance progress on existing and future NIH-funded research. Public Health Relevance: Access to Surface Plasmon Resonance will accelerate existing and open up new research applications across the medical campus of UM. The impacted projects range from very basic to applied research with immediate translational potential. We therefore expect a highly synergistic impact on studies aimed at improving human health.

描述（由申请人提供）：迈阿密大学米勒医学院的六个基础研究部门开展了广泛的基础科学研究，具有很强的转化成分。综合癌症中心（有助于维护拟议的 SPR 设备）等跨部门结构促进了基础研究人员与临床研究小组之间的互动。在这组研究人员中（包括来自邻近的佛罗里达斯克里普斯研究所的一名用户），强烈需要能够以小样本量进行高灵敏度和无标记分子相互作用研究的模式。在过去的十年中，表面等离子共振即使不是占主导地位，也已成为此类应用的主要分析方法，特别是近年来其灵敏度的提高（现在包括小分子分析物的无标记结合研究）和多路复用和自动化测量的能力。这使得能够以最少的样本量高效地进行广泛的研究。我们对 SPR 能力的高需求与在 UM 数百英里半径内没有任何此类开放访问的设备形成鲜明对比。 SPR 能力的缺乏迫使研究人员充其量只能依赖灵敏度较低、信息量较少的方法。在某些情况下，这种新型小分子先导化合物的无标记结合，没有其他选择可用。在其他情况下（抗体优化），外包是目前唯一的选择 拟议购买 Biacore T200 将为 UM 校园建立急需的 SPR 功能。该仪器将安置在受监督的仪器设施中，并通过机构支持进行维护。特色应用包括治疗用抗体的表征和优化、现有或从头合成的小分子量药物的评估、作为替代方法的 ITC 和 SPR 之间热力学结合特性的详细比较、DNA 上多蛋白复合物的组装、 SELEX 衍生核酸适体针对靶蛋白的表征，以及血清样品中分析物浓度的检测，以及几个强调 SPR 在弱相互作用研究中的优势的项目 所有应用都属于 Biacore 的能力范围T200 和 SPR 的可用性肯定会促进现有和未来 NIH 资助的研究取得进展。 公共卫生相关性：使用表面等离子共振将加速整个密歇根大学医学园区的现有研究应用并开辟新的研究应用。受影响的项目范围从非常基础的研究到具有直接转化潜力的应用研究。因此，我们期望对旨在改善人类健康的研究产生高度协同影响。