Lung Biological Specimens Core

肺生物标本核心

• 批准号: 8380132
• 负责人: Anne I. Sperling
• 金额: $ 17.38万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8380132
• 关键词: Affect; Asthma; Biocompatible Materials; Biological; Biological Assay; Biopsy; Blood; Blood Cells; Blood specimen; Bronchoalveolar Lavage; Bronchoalveolar Lavage Fluid; Bronchoscopy; CD4 Positive T Lymphocytes; Cell Separation; Cells; Clinical; Clinical Trials Data Monitoring Committees; Collecting Cell; Collection; Core Facility; DNA; Databases; Doctor of Philosophy; Ensure; Epithelial Cells; Familiarity; Flow Cytometry; Gene Expression; Genes; Genetic; Genotype; HLA G antigen; Immune; Immunohistochemistry; Infiltration; Label; Liquid substance; Lung; Lymphocyte; Lymphocyte Subset; Medicine; MicroRNAs; Mononuclear; Morbidity - disease rate; National Institute of Allergy and Infectious Disease; Pathogenesis; Patient Recruitments; Performance; Peripheral Blood Mononuclear Cell; Plasma; Process; Quality Control; RNA; RNA analysis; Regulation; Reporting; Research; Research Subjects; Role; Sampling; Smooth Muscle Myocytes; Specimen; Suppressor-Effector T-Lymphocytes; T-Lymphocyte; United States; Universities; Work; airway epithelium; airway inflammation; chemokine; cytokine; data management; eosinophil; established cell line; experience; laser capture microdissection; meetings; professor; programs; receptor; research study; respiratory smooth muscle; synthetic polymer Bioplex; tissue processing

The Lung Biospecimens Core will provide our AADCRC Program with processing of all blood and lung specimens to be collected for each project. This includes all tissue processing, isolation of cells from blood and bronchoalveolar lavage fluid. Isolation of DNA, RNA and miRNA, and performance of bioplex cytokine and chemokine assays. The Core is led by Anne Sperling, PhD, Associate Professor of Medicine, who has extensive experience in the administration of university-wide core facilities and deep familiarity with all the assays and isolations to be performed by this Core. A senior research technologist performs all assays and isolations and maintains rigorous quality control and record-keeping. Timely isolation and storage of biological materials is of the utmost importance in the successful completion of each of the projects in this program. This dedicated Core will meet these obligations.

肺部生物测量核心将为我们的AADCRC计划提供所有血液和肺的处理 为每个项目收集标本。这包括所有组织加工，从血液中隔离细胞 和支气管肺泡灌洗液。 DNA，RNA和miRNA的分离，以及生物膜细胞因子的性能和 趋化因子测定。 核心由医学副教授安妮·斯佩林（Anne Sperling）领导，他在 全大学核心设施的管理以及对所有分析和隔离的熟悉 可以通过此核心执行。一名高级研究技术专家执行所有测定和隔离，并保持 严格的质量控制和记录保存。 及时的隔离和存储生物材料在成功完成中至关重要 该计划中的每个项目。这个专门的核心将符合这些义务。