Request for a Spectral Confocal Microscope

索取光谱共焦显微镜

基本信息

批准号：
7793953
负责人：
THOMAS C RICH
金额：
$ 39.74万
依托单位：
UNIVERSITY OF SOUTH ALABAMA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-06 至 2011-05-05
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Eleven NIH-funded investigators and one junior investigator with a pending NIH application are seeking support under the NCRR Shared Instrumentation Grant Program to obtain a Nikon A1 laser scanning confocal microscope. The Nikon microscope is justified because the two other confocal microscopes available at the University of South Alabama - a nine-year-old Leica TCS SP2 spectral confocal microscope and a five year old PerkinElmer Ultraview RS-3 spinning disk confocal microscope - are heavily used by thirty labs and more than fifty individuals. As such, access to these microscopes has become a rate-limiting step in conducting research. This limitation has worsened because increased usage and age have led to component failure and subsequent down time of the Leica system. In addition, neither microscope is capable of obtaining high resolution confocal images of preparations in environmental chambers or making live-cell, time lapse measurements of multiple fluorophores. The steering committee of the BioImaging Core Facility has identified the Nikon A1 laser scanning confocal microscope as the system best suited for our needs, because of its high acquisition speed, high-spatial resolution, incorporation of an acousto-optical tunable filter to control laser intensity, and ability to simultaneously acquire spectra divided into 32 separate wavelength bins of 2.5, 5, or 10 nm, and Nikon's excellent customer service at the University of South Alabama. The Nikon microscope will support a group of investigators conducting research in the areas of Lung Endothelial Biology, Vascular Smooth Muscle Biology, and Cancer Biology. As such, the work is highly relevant to understanding the underlying factors contributing to hypertension, vascular disease, COPD, and breast cancer, as well as the development of treatments for these conditions. The system will be housed in the BioImaging Core Facility with the two current confocal microscopes. The USA College of Medicine has committed to refurbish space, and to provide financial support for a three-year extended service contract with Nikon, and a Ph.D. level manager to run the BioImaging Core Facility. This individual will assist researchers with the use of the Nikon system, as well as other aspects of experimental design. The BioImaging Core Facility will ensure appropriate use and sharing of the system. The addition of the new generation confocal microscope will greatly expand the imaging capabilities available to NIH-funded researchers present on University of South Alabama campus.

描述（由申请人提供）： 11 名 NIH 资助的研究人员和一名正在等待 NIH 申请的初级研究人员正在 NCRR 共享仪器资助计划下寻求支持，以获得一台 Nikon A1 激光扫描共聚焦显微镜。尼康显微镜是合理的，因为南阿拉巴马大学提供的另外两台共焦显微镜 - 一台已使用 9 年的 Leica TCS SP2 光谱共焦显微镜和一台已使用 5 年的 PerkinElmer Ultraview RS-3 转盘共焦显微镜 - 被大量使用三十个实验室和五十多人。因此，使用这些显微镜已成为进行研究的限制步骤。这种限制变得更加严重，因为使用量的增加和老化导致了徕卡系统的组件故障和随后的停机时间。此外，这两种显微镜都无法获得环境室中制剂的高分辨率共焦图像或对多个荧光团进行活细胞延时测量。生物成像核心设施的指导委员会已将 Nikon A1 激光扫描共焦显微镜确定为最适合我们需求的系统，因为它具有高采集速度、高空间分辨率、并结合声光可调谐滤波器来控制激光强度，并能够同时获取分为 32 个独立波长档（2.5、5 或 10 nm）的光谱，以及尼康在南阿拉巴马大学提供的卓越客户服务。尼康显微镜将支持一组研究人员在肺内皮生物学、血管平滑肌生物学和癌症生物学领域进行研究。因此，这项工作与了解导致高血压、血管疾病、慢性阻塞性肺病和乳腺癌的潜在因素以及开发这些疾病的治疗方法高度相关。该系统将安装在生物成像核心设施中，并配有两台现有的共焦显微镜。美国医学院已承诺翻新空间，并为与尼康签订的三年延长服务合同提供财政支持，并为博士生提供资助。运行生物成像核心设施的级别经理。此人将协助研究人员使用尼康系统以及实验设计的其他方面。生物成像核心设施将确保系统的适当使用和共享。新一代共焦显微镜的加入将极大地扩展南阿拉巴马大学校园内由 NIH 资助的研究人员的成像能力。