ZEISS Spinning Disc/LSM700 Point Scanning Confocal Microscope with Incubation Sys

蔡司转盘/LSM700 点扫描共焦显微镜，带孵化系统

• 批准号: 7794517
• 负责人: CRISTINA Maria FERNANDEZ-VALLE
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CENTRAL FLORIDA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794517
• 关键词: Area; Award; Biomedical Research; Cardiovascular Diseases; Cells; Communicable Diseases; Computer software; Contract Services; Core Facility; Dyes; Faculty; Fingerprint; Florida; Fluorescence; Funding; Goals; Image; Lasers; Life; Malignant Neoplasms; Medicine; Microscope; Minor; Neurobiology; Nuclear; Recruitment Activity; Request for Proposals; Research; Research Personnel; Scanning; Schools; Science; System; Three-Dimensional Imaging; Time; United States National Institutes of Health; Universities; bioimaging; cellular imaging; college; data acquisition; instrument; photoactivation; programs; research study

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Zeiss Spinning Disc/LSM 700 Point Scanning Confocal Microscope Combination System for the Bioimaging Core Facility of the Burnett School Biomedical Sciences in the newly-formed College of Medicine at University of Central Florida (UCF). The system includes an integrated Zeiss LSM 700 laser scanning confocal microscope, with a cell-observer platform and a CSU-X1 spinning disc system. This instrument allows users to perform live cell time-lapse imaging, 3D imaging, multiple fluorescence and colocalization analyses, nuclear localization, emission fingerprinting, DIC, FRAP, FLIP, photoactivation and photoconversion experiments. The unique capabilities of LSM 700 include variable secondary dichroic beam splitter, FixGate main beam splitter, laser life extender, and integrated software that controls data acquisition and analysis of multiple parameters. There is a critical need for this instrument because there is only one common use confocal microscope available at UCF and in the Central Florida area. It is a Zeiss LSM 510 scanning confocal microscope that was purchased in 2000 and is the sole instrument in the existing Bioimaging Core. This microscope stand has become obsolete and will not be supported by a Zeiss service contract as of September 2009. Furthermore, the current system does not have the capability of live cell imaging or UV excitation of nuclear dyes such as DAPI. The 8 major users of this proposal have 12 NIH funded R01 projects (2 additional R01 will begin in 2009) focused on neurobiology, cardiovascular diseases, infectious diseases, and cancer. Each award has experiments that require confocal and live imaging. In addition, there are 4 minor users who have NIH-funded projects. The purchase of the microscope system will help sustain existing NIH-funded research programs and will help recruit additional outstanding biomedical researchers. This will help the unit fulfill its strategic goal defined in 2003 to recruit 34 new faculty to build a well-funded nationally recognized biomedical research program at UCF.

描述（由申请人提供）：本提案请求资金购买蔡司转盘/LSM 700 点扫描共焦显微镜组合系统，用于中佛罗里达大学新成立的医学院伯内特学院生物医学科学的生物成像核心设施（中佛罗里达大学）。该系统包括集成的 Zeiss LSM 700 激光扫描共焦显微镜、细胞观察平台和 CSU-X1 旋转盘系统。该仪器允许用户执行活细胞延时成像、3D 成像、多重荧光和共定位分析、核定位、发射指纹、DIC、FRAP、FLIP、光激活和光转换实验。 LSM 700 的独特功能包括可变二次二向色分束器、FixGate 主分束器、激光寿命延长器以及控制数据采集和多个参数分析的集成软件。由于 UCF 和佛罗里达州中部地区只有一台常用共焦显微镜，因此对这种仪器的需求非常迫切。它是 2000 年购买的 Zeiss LSM 510 扫描共焦显微镜，是现有生物成像核心中的唯一仪器。该显微镜支架已过时，自 2009 年 9 月起将不再获得蔡司服务合同的支持。此外，当前系统不具备活细胞成像或 DAPI 等核染料的 UV 激发功能。该提案的 8 个主要用户拥有 12 个 NIH 资助的 R01 项目（另外 2 个 R01 项目将于 2009 年开始），重点关注神经生物学、心血管疾病、传染病和癌症。每个奖项都有需要共焦和实时成像的实验。此外，还有 4 个未成年用户拥有 NIH 资助的项目。购买显微镜系统将有助于维持现有的 NIH 资助的研究项目，并将有助于招募更多优秀的生物医学研究人员。这将有助于该部门实现 2003 年确定的战略目标，即招募 34 名新教员，在中佛罗里达大学建立一个资金充足的国家认可的生物医学研究项目。

项目成果

In-fiber production of polymeric particles for biosensing and encapsulation.

用于生物传感和封装的聚合物颗粒的纤维内生产。

• 发表时间: 2013-09-24
• 影响因子: 11.1
• 作者: Kaufman, Joshua J;Ottman, Richard;Tao, Guangming;Shabahang, Soroush;Banaei, Esmaeil;Liang, Xiangdong;Johnson, Steven G;Fink, Yoel;Chakrabarti, Ratna;Abouraddy, Ayman F
• 通讯作者: Abouraddy, Ayman F