Core C - Functional Genomics

核心 C - 功能基因组学

• 批准号: 8572233
• 负责人: PETER S RABINOVITCH
• 金额: $ 28.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8572233
• 关键词: Age; Aging; Biological; Biology of Aging; Cellular Assay; Communities; Data; Funding; Gene Expression; Genetic; Informatics; Institution; Knock-out; Longevity Pathway; Modeling; Mutation; Office of Administrative Management; Organ; Pathway interactions; Physiological; Pilot Projects; Post-Translational Protein Processing; Proteins; Proteomics; Research Personnel; Resources; Shock; Technology; Transgenic Animals; Translations; Universities; Washington; animal model development; comparative; data sharing; functional genomics; mouse model; programs; protein degradation; research and development; transcriptomics

This application is for renewal of the Nathan Shock Center of Excellence in the Basic Biology of Aging at the University of Washington and affiliated institutions. This Center has over the past 15 years provided resources in support of our large community of investigators that are funded to study basic biological mechanisms of aging. We continue to build upon a scientific theme that became established in the past funding cycle, in which comparative gerontologic study informed us of conserved genetic pathways of longevity determination (the TOR pathway is an example of one such). The three proposed Research Resources Core components are well suited to assist in such efforts: 1) Transgenic Animal Model Development; 2) Functional Assessment; 3) Functional Genomics. The Transgenic Animal Model Development Resource Core continues to pursue a focus on inducible gene expression and knockout models, so that organ and age-specific effects of genetic alterations can be assessed. The Functional Assessment Core builds upon technical resources that have been gathered during the past funding period, such that we can now provide investigators with a broad and informative range of physiologic and cellular assays, including those that are most relevant to assessment of the healthspan of mouse models. The Functional Genomics Core provides expert assistance with state of the art technologies for transcriptomics and proteomics. Consistent with our theme, it includes a focus on translational state, protein turnover and post-translational modification. The Research Development Core will continue to support pilot study projects in the basic biology of aging. The Program Enrichment Core supports administrative management, an external advisory panel, a program of courses and seminars and data sharing and dissemination.

该应用是为了续签内森的卓越卓越中心的衰老基本生物学中心 华盛顿大学和附属机构。该中心在过去的15年中有 支持我们大型研究人员社区的资源，这些调查员被资助用于研究基本生物学 衰老机制。我们继续以过去建立的科学主题为基础 筹资周期，比较的老年学研究向我们告知了我们的保守遗传途径 寿命确定（TOR途径就是这样的例子）。提出的三项研究 Resources核心组成部分非常适合进行此类努力：1）转基因动物模型 发展; 2）功能评估； 3）功能基因组学。转基因动物模型 发展资源核心继续专注于诱导基因表达和敲除 模型，以便可以评估遗传改变的器官和年龄特异性效应。功能 评估核心基于过去筹资期收集的技术资源， 因此，我们现在可以为研究人员提供广泛且信息丰富的生理和细胞范围 测定法，包括与评估小鼠模型的评估最相关的测定法。这 功能基因组学核心为转录组学的最先进技术提供了专家帮助 和蛋白质组学。与我们的主题一致，它的重点是转化状态，蛋白质流失和 翻译后修改。研究开发核心将继续支持试点研究项目 在衰老的基本生物学中。该计划的丰富核心支持行政管理 外部咨询小组，课程，研讨会以及数据共享和传播计划。