DETERMINATION OF THE STRUCTURE OF VITAMIN K EPOXIDE REDUCTASE (VKOR)

维生素 K 环氧还原酶 (VKOR) 结构的测定

• 批准号: 7955145
• 负责人: Tom A Rapoport
• 金额: $ 2.14万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955145
• 关键词: Bacteria; Blood Coagulation Factor; Blood coagulation; Computer Retrieval of Information on Scientific Projects Database; Disulfides; Funding; Goals; Grant; Homologous Gene; Hydroquinones; Institution; Light; Proteins; Research; Research Personnel; Resources; Roentgen Rays; Source; Structure; United States National Institutes of Health; Vitamin K; Warfarin; carboxylate; cofactor; hydroquinone; inhibitor/antagonist; reduced vitamin K; structural biology; vitamin K epoxide reductase; vitamin K1 oxide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. VKOR reduces vitamin K epoxide to the hydroquinone form of vitamin K. Reduced vitamin K is a cofactor for a carboxylase that gamme-carboxylates several proteins, including several blood coagulation factors. VKOR is the target for warfarin, the major blood coagulation inhibitor. Homologs of VKOR exist in several bacteria and have recently been shown to be involved in disulfide bridge formation. The goal of this project is to determine an Xray structure of one of these homologs. The structure would likely shed light on the mechanism of disulfide bridge formation and warfarin inhibition.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 VKOR 将维生素 K 环氧化物还原为维生素 K 对苯二酚形式。还原的维生素 K 是羧化酶的辅助因子，该酶可对多种蛋白质（包括多种凝血因子）进行 γ 羧化。 VKOR 是主要凝血抑制剂华法林的靶标。 VKOR 的同系物存在于多种细菌中，最近被证明参与二硫键的形成。该项目的目标是确定这些同系物之一的 X 射线结构。该结构可能有助于揭示二硫键形成和华法林抑制的机制。