Research Support Core: Inhalation Toxicology Core

研究支持核心：吸入毒理学核心

• 批准号: 8249996
• 负责人: LARRY W ROBERTSON
• 金额: $ 13.42万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8249996
• 关键词: Acute; Aerosols; Air; Air Conditioning; Animal Experimentation; Animal Model; Animals; Apical; Area; Autopsy; Binding; Biological; Biological Assay; Blood; Cell Line; Chemicals; Cities; Control Animal; Electronics; Environmental Health; Environmental Wind; Epithelial Cells; Equipment; Experimental Designs; Exposure to; Filtration; Goals; Health; Human; Humidity; Individual; Industry; Inhalation Exposure; Inhalation Toxicology; Laboratories; Laboratory Animals; Life; Lung; Measures; Metabolic; Metabolism; Methods; Molecular Weight; Monitor; Movement; Mus; Neurologic; Nose; Phase; Poison; Pollution; Polychlorinated Biphenyls; Population; Protocols documentation; Rain; Rattus; Relative (related person); Research Personnel; Research Support; Risk Assessment; Rodent; Science; Seasons; Secure; Sentinel; Services; Site; Social Welfare; Soil; Solubility; Source; System; Temperature; Time; Tissues; Toxic Actions; Toxic effect; Toxicology; Water; Work; airway epithelium; alveolar epithelium; animal facility; design; exhaust; health science research; innovation; landfill; novel strategies; operation; particle; planetary Atmosphere; pressure; programs; quality assurance; remediation; reproductive; response; urban area; vapor; vaporization

Inhalation exposure to semi-volatile PCBs in industrial cities, near the Great Lakes, in areas where dredging is occurring and at remediation sites has the potential to cause neurological, reproductive, and immunological toxicity in humans and animals. The Inhalation Toxicology Core was established with the primary goal to serve the projects and other research support cores with state-of-the-art animal facilities and rigorously controlled inhalation exposures to realistic mixtures of airborne PCB congeners. The specific aims of this Core are: Aim 1) Generate controlled PCB atmospheres in exposure chambers and collect airborne PCBs resulting from vaporization of common industrial PCB mixtures (supports Projects 1, 2, 3, 5 and the Analytical and Synthesis Cores); Aim 2) Perform acute, subacute and subchronic inhalation exposures of laboratory animals to defined mixtures of atmospheric PCBs and to individual congeners, (supports Projects 1 and 2); Aim 3) Provide a rodent vivarium for experimental animals used for the isbrp and execute a quality assurance program to monitor their health and welfare (supports Projects 1, 2, and 3); Aim 4) Perform necropies on exposed, control and sentinel rodents and distribute tissues to project investigators in the isbrp (supports Projects 1,2, and 3). The Inhalation Toxicology Core exists within the Pulmonary Toxicology Facility (PTF) of the Environmental Health Sciences Research Center and consists of 6,000 ft2 of contiguous laboratories and offices. The PTF is nationally recognized for innovative animal models and exposure delivery systems. A full array of inhalation toxicology, aerosol science, and bioassay services are rendered. The Inhalation Toxicology Core is well equipped for generating and quantifying PCB vapors or aerosols; for performing nose-only inhalation exposures of rats and mice in acute, subacute or subchronic protocols; and for determining the degree of exposure and response. A new aim in this renewal is: Aim 5) Perform airborne exposures to primary alveolar and airway epithelia and epithelial cell lines in Transwells with an apical air interface using vapor phase and particle bound PCB congeners. This will bring to the program a new approach for studying toxic effects of airborne PCBs on epithelial cells.

在大湖区附近，在发生疏pc的地区，在修复地点发生的地区，在工业城市中吸入的半挥发性PCB有可能引起人类和动物的神经学，生殖和免疫学毒性。吸入毒理学核心是建立的，其主要目标是为项目和其他研究支持核心提供最先进的动物设施，并严格控制着对机载PCB同源物的现实混合物的严格控制的吸入暴露。该核心的具体目的是：目标1）在暴露室中产生受控的PCB大气，并收集由常见工业PCB混合物蒸发而导致的空气中PCB（支持项目1、2、3、5和分析和合成核心）；目标2）对实验动物进行急性，亚急性和亚少生吸入暴露，以确定大气PCB和单个同类物的混合物（支持项目1和2）；目标3）为用于ISBRP的实验动物提供啮齿动物的啮齿动物，并执行质量保证计划以监控其健康和福利（支持项目1、2和3）；目标4）对暴露，控制和哨兵啮齿动物进行坏死，并将组织分配给ISBRP的项目研究人员（支持项目1,2和3）。在环境健康科学研究中心的肺毒理学设施（PTF）内，吸入毒理学核心存在，由6,000英尺的连续实验室和办公室组成。 PTF因创新的动物模型和暴露传递系统而在全国范围内得到认可。一系列吸入毒理学，气溶胶 科学和生物测定服务是提供的。吸入毒理学核心有足够的能力产生和量化PCB蒸气或气溶胶。用于在急性，亚急性或亚慢性方案中对大鼠和小鼠的仅鼻子吸入暴露；并确定暴露程度和反应程度。此续签的一个新目标是：目标5）使用蒸气相和粒子绑定的PCB同源物，在带顶空气界面的Transwell中对原发性肺泡和气道上皮和上皮细胞系进行空中暴露。这将为该计划带来一种新的方法，用于研究空气中PCB对上皮细胞的毒性作用。