RNA STRUCTURE AND FUNCTION

RNA结构和功能

• 批准号: 8361700
• 负责人: Navtej Singh Toor
• 金额: $ 0.55万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361700
• 关键词: Catalytic Domain; Catalytic RNA; Complex; Crystallography; Eukaryota; Excision; Exons; Funding; Grant; Human Genome; Introns; Ligation; National Center for Research Resources; Nuclear; Principal Investigator; Process; RNA; RNA Splicing; Research; Research Infrastructure; Resources; Retroelements; Source; Spliceosomes; Structure; United States National Institutes of Health; cost; genetic element; mRNA Precursor; protein complex; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our research involves studying the structure and function of large catalytic RNAs and RNA-protein complexes using x-ray crystallography. Specifically, we are studying group II introns, which are a class of self-splicing ribozymes that can also function as mobile retroelements. Group II introns undergo a two-step splicing process that leads to their excision from pre-mRNAs and results in the ligation of the adjacent exons. They are also thought to be the ancestors of the spliceosomal introns and non-LTR retroelements, which together comprise ~50% of the human genome. The group II intron and the spliceosome also share a conserved, catalytic core. The spliceosome is a multi-megadalton complex which is responsible for excising nuclear introns in eukaryotes. Through studying group II introns, we can also gain an understanding of these more evolutionarily advanced genetic elements.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们的研究涉及使用X射线晶体学研究大型催化RNA和RNA-蛋白质复合物的结构和功能。 具体而言，我们正在研究II组内含子，这些内含子是一类自剪式核酶，也可以用作移动恢复元素。 II组内含子经历了两步剪接过程，从而导致其从MRNA中切除并导致相邻外显子的连接。 它们也被认为是剪接体内含子和非LTR恢复元素的祖先，共同占人类基因组的50％。 II组内含子和剪接体还具有保守的催化核心。 剪接体是一种多核综合体，负责在真核生物中切除核内含子。 通过研究II组内含子，我们还可以了解这些更进化的遗传元素。