STRUCTURAL STUDIES OF NATIVE AND DESIGNED ALPHA HELICAL COILED COILS

原生和设计的 α 螺旋线圈的结构研究

• 批准号: 8361626
• 负责人: AMY E KEATING
• 金额: $ 0.16万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361626
• 关键词: Coiled-Coil Domain; Computer Analysis; Funding; Genetic Transcription; Grant; Human; Measurement; Modeling; National Center for Research Resources; Peptides; Principal Investigator; Proteins; Proteome; Research; Research Infrastructure; Resources; Source; Specificity; Structure; United States National Institutes of Health; Yeasts; combinatorial; cost; design; dimer; interest; programs; spindle pole body; structural biology; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Research in the Keating lab aims to understand determinants of structural specificity and interaction specificity in alpha-helical coiled coils, a common interaction motif in the proteome. We apply an integrated program of experimental measurement, computational analysis, and structure determinantion. Targets of particular interest include bZIP transcription factors and coiled coils of the yeast spindle pole body. For the bZIPs, combinatorial interactions that regulate transcription are made via coiled-coil dimers. We are studying both native and synthetic coiled-coil peptides that interact with human bZIPs by forming coiled-coil dimers. Proteins of the spindle pole body are highly enriched in predicted coiled-coil domains, and we are characterizing these as part of an effort to build models of the overall structure of the very large spindle pole body assembly.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 Keating 实验室的研究旨在了解 α 螺旋卷曲线圈（蛋白质组中常见的相互作用基序）的结构特异性和相互作用特异性的决定因素。我们应用实验测量、计算分析和结构测定的综合程序。特别感兴趣的靶标包括 bZIP 转录因子和酵母纺锤体的卷曲线圈。对于 bZIP，调节转录的组合相互作用是通过卷曲螺旋二聚体实现的。我们正在研究天然和合成的卷曲螺旋肽，它们通过形成卷曲螺旋二聚体与人类 bZIP 相互作用。纺锤体极体的蛋白质在预测的卷曲螺旋结构域中高度富集，我们将这些特征描述为构建超大型纺锤体极体组件整体结构模型的努力的一部分。