STRUCTURES OF RIBOSOME-BOUND MODIFIED TRNAS

核糖体结合修饰的 TRNAS 的结构

• 批准号: 8361726
• 负责人: PAUL F AGRIS
• 金额: $ 2.01万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361726
• 关键词: Amino Acyl-tRNA Synthetases; Anticodon; Binding; Cellular biology; Codon Nucleotides; Funding; Grant; HIV; Human; Methods; Molecular Biology; National Center for Research Resources; Nucleic Acids; Nucleosides; Play; Positioning Attribute; Principal Investigator; Protein Biosynthesis; Research; Research Infrastructure; Resources; Reverse Transcription; Ribosomal Frameshifting; Ribosomes; Role; Source; Stable Isotope Labeling; Structure; Structure-Activity Relationship; Transfer RNA; United States National Institutes of Health; cost; programs; structural biology; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Structure-function relationships of nucleic acids, such as that of tRNA in protein synthesis, are fundamental to all cell and molecular biology. In order to probe the structure-function relationships of RNAs as potential targets or tools, we have developed methods for the introduction of native, non-natural, and stable isotope labeled nucleosides. We have found that modified nucleosides in tRNA play an important structural and functional role both within the tRNA molecules and in tRNA anticodon recognition of select codons at the wobble position. Modified nucleosides alter codon "wobble", enhance ribosome binding, explain programmed translational frameshifting, are determinants for aminoacyl-tRNA synthetase recognition, and are involved in human immunodeficiency virus selection of a specific human tRNA to prime reverse transcription.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 核酸的结构-功能关系，例如蛋白质合成中 tRNA 的结构-功能关系，是所有细胞和分子生物学的基础。为了探索作为潜在靶标或工具的 RNA 的结构功能关系，我们开发了引入天然、非天然和稳定同位素标记核苷的方法。我们发现，tRNA 中的修饰核苷在 tRNA 分子内以及在 tRNA 反密码子识别摆动位置的选定密码子中发挥着重要的结构和功能作用。修饰核苷改变密码子“摆动”，增强核糖体结合，解释程序化翻译移码，是氨酰基-tRNA合成酶识别的决定因素，并参与人类免疫缺陷病毒选择特定人类tRNA以启动逆转录。