MODIFIED NUCLEOSIDE STRUCTURE-FUNCTION RELATIONS--TRNA

修饰核苷结构-功能关系--TRNA

• 批准号: 6291906
• 负责人: PAUL F AGRIS
• 金额: $ 4.65万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-01 至 2001-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291906
• 关键词: biophysics; chemical stability; chemical structure function; circular dichroism; conformation; divalent cations; genetic translation; intermolecular interaction; ionic bond; magnesium; nuclear magnetic resonance spectroscopy; nucleic acid chemical synthesis; nucleic acid sequence; nucleic acid structure; nucleoside analog; oligonucleotides; ribosomal RNA; ribosomes; solutions; structural biology; synthetic nucleotide; transfer RNA

DESCRIPTION: The long term objective is to apply the physicochemical properties that modified nucleosides contribute to nucleic acids to the design of pharmaceutical agents that inhibit translation. The research is focused on understanding the contributions of several modified nucleosides to RNA structural stability, ion binding, dynamics, folding, RNA-RNA interactions, and RNA-protein interactions. In particular, the contributions of modified nucleosides to the properties, structures, and functions of various domains from tRNA will be studied using CD, NMR, and biochemical methods. The information will be used to design inhibitors of translation.

描述：长期目标是应用物理化学 修饰核苷有助于核酸的特性 抑制翻译的药物制剂的设计。 研究 重点是了解几个修改的贡献 核苷与RNA结构稳定性、离子结合、动力学、折叠、 RNA-RNA 相互作用，以及 RNA-蛋白质相互作用。 特别是， 修饰核苷对性质、结构和作用的贡献 将使用 CD、NMR、 和生化方法。 该信息将用于设计 翻译抑制剂。