N-LINKED GLYCOSYLATION SITE MAPPING OF HIV-1 GP120

HIV-1 GP120 的 N 联糖基化位点定位

• 批准号: 8363095
• 负责人: Parastoo Azadi
• 金额: $ 0.61万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363095
• 关键词: Antibodies; Binding; Complex; Digestion; Funding; Grant; HIV Envelope Protein gp120; HIV-1; Link; Maps; National Center for Research Resources; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Site; Source; Technology; United States National Institutes of Health; cost; glycosylation; interest; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Dr. Zhou's group is interested in the binding interaction of VRC05 antibody that recognizes glycans on HIV-1 gp120. The HIV-1 gp120-VRC05 complex was prepared by mixing glycosylated gp120 and VRC05 Fab, and then treated with EndoH to remove non-interacting glycans. The presence of VRC05 protected few glycans from EndoH digestion. Those glycans are critical for VRC05 binding to HIV-1 gp120. In this study, we took the part of investigating the site of glycosylation of this protein complex.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Zhou博士的小组对在HIV-1 GP120上识别聚糖的VRC05抗体的结合相互作用感兴趣。 HIV-1 GP120-VRC05复合物是通过混合糖基化的GP120和VRC05 Fab制备的，然后用EndoH处理以去除非相互作用的聚糖。 VRC05的存在保护了很少的聚糖免受胚胎消化的影响。 这些聚糖对于与HIV-1 GP120结合的VRC05至关重要。 在这项研究中，我们竭尽全力研究该蛋白质复合物的糖基化部位。