CHARACTERIZATION OF TRICHOMONAD LIPOPHOSPHOGLYCAN

毛滴虫脂磷酸聚糖的表征

• 批准号: 8365543
• 负责人: BIBHUTI N. SINGH
• 金额: $ 1.7万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365543
• 关键词: Acids; Acquired Immunodeficiency Syndrome; Biological; Biology; Cattle; Data; Enzymes; Epithelial Cells; Fetus; Funding; Grant; Human; Infection; Investigation; Mass Spectrum Analysis; Medicine; Methods; National Center for Research Resources; Oligosaccharides; Parasites; Patients; Phospholipase C; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Sequence Determination; Sexually Transmitted Diseases; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; System; Trichomonas vaginalis; Trifluoroacetic Acid; United States National Institutes of Health; Up-Regulation; Variant; beta-Galactosidase; cost; cytokine; human female; keratan-sulfate endo-1,4-b-galactosidase; lipophosphonoglycan; mass spectrometer; reproductive; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Trichomonad parasites, T. vaginalis (Tv) and T. foetus (Tf), cause major sexually transmitted diseases in human and cattle, respectively, and may predispose human patients to AIDS infection. BNS et al. purified lipophosphoglycan from both parasites an treated them with mild acid (trifluoroacetic acid) and different enzymes (endo-beta-galactosidase, another beta-galactosidase, phospholipase C) in order to obtain oligosaccharide structures short enough for MS/MS characterization. The fractions from Tv were initially characterized by MALDI-TOF MS, then the oligosaccharide portions were reduced and permethylated before analysis by LC-MS/MS, using the QoTOF mass spectrometer or the LTQ-Orbitrap MS system. A variety of sugars assigned to different structural motifs was identified. Many structures have been assigned and further data interpretation and investigation is ongoing. Biological characterization has shown that the T. vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. Several new samples of the lipophosphoglycan have been prepared for variants of the T. vaginalis. The structures are being determined by MALDI- and ESI-MS. LacNAc repeats are visible even in the MALDI-TOF mass spectra. Methods developed at the MS Resource for ETD of Glycans are being employed for sequence determinations.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Trichomonad寄生虫，T。Vaginalis（TV）和T.胎儿（TF），分别引起人类和牛的主要性传播疾病，并且可能使人类患者诱发艾滋病感染。 BNS等。从两种寄生虫和用温和酸（三氟乙酸）和不同的酶（内抗β-半乳糖苷酶，另一种β-半乳糖苷酶，磷脂酶C）处理的纯化的脂磷酸聚糖，以获得足够短的寡糖糖结构，足以获得MS/MS的特征。最初，来自电视的馏分以MALDI-TOF MS进行表征，然后使用QOTOF质谱仪或LTQ-Orbitrap MS系统，在通过LC-MS/MS分析之前，在LC-MS/MS进行分析之前，将寡糖部分减少并氯化。确定了分配给不同结构基序的各种糖。已经分配了许多结构，并进行了进一步的数据解释，并且正在进行调查。生物学表征表明，阴道脂肪磷酸t. t. t. phipophoglycan触发了人类雌性生殖道上皮细胞对细胞因子的选择性上调。为阴道的变体准备了几种新脂蛋白粉的新样品。这些结构由马尔代 - 和ESI-MS确定。即使在MALDI-TOF质谱中，LACNAC重复也可以看到。在MS资源中为聚糖的MS资源开发的方法已用于序列确定。