CHARACTERIZATION OF TRICHOMONAD LIPOPHOSPHOGLYCAN

毛滴虫脂磷酸聚糖的表征

• 批准号: 8170911
• 负责人: BIBHUTI N. SINGH
• 金额: $ 1.02万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170911
• 关键词: Acids; Acquired Immunodeficiency Syndrome; Biological; Cattle; Computer Retrieval of Information on Scientific Projects Database; Data; Enzymes; Epithelial Cells; Fetus; Funding; Germany; Grant; Human; Infection; Institution; International; Investigation; Manuscripts; Mass Spectrum Analysis; Natural graphite; Oligosaccharides; Parasites; Patients; Phospholipase C; Publishing; Research; Research Personnel; Resources; Sexually Transmitted Diseases; Source; Structure; System; Trichomonas vaginalis; Trifluoroacetic Acid; United States National Institutes of Health; Up-Regulation; beta-Galactosidase; cytokine; human female; keratan-sulfate endo-1,4-b-galactosidase; lipophosphonoglycan; mass spectrometer; posters; reproductive; sugar; symposium

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Trichomonad parasites, T. vaginalis (Tv) and T. foetus (Tf), cause major sexually transmitted diseases in human and cattle, respectively, and may predispose human patients to AIDS infection. BNS et al. purified lipophosphoglycan from both parasites an treated them with mild acid (trifluoroacetic acid) and different enzymes (endo-beta-galactosidase, another beta-galactosidase, phospholipase C) in order to obtain oligosaccharide structures short enough for MS/MS characterization. The fractions from Tv were initially characterized by MALDI-TOF MS, then the oligosaccharide portions were reduced and permethylated before analysis by LC-MS/MS, using a graphite column and a QoTOF mass spectrometer. or the LTQ-Orbitrap MS system. A variety of sugars assigned to different structural motifs was identified. Many structures have been assigned and further data interpretation and investigation is ongoing. Biological characterization has shown that the T. vaginalis Lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. Two manuscripts describing results from this project have been published recently and a poster was presented at the 2009 International Mass Spectrometry Conference in Bremen, Germany.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 Trichomonad寄生虫，T。Vaginalis（TV）和T.胎儿（TF），分别引起人类和牛的主要性传播疾病，并且可能使人类患者诱发艾滋病感染。 BNS等。从两种寄生虫和用温和酸（三氟乙酸）和不同的酶（内抗β-半乳糖苷酶，另一种β-半乳糖苷酶，磷脂酶C）处理的纯化的脂磷酸聚糖，以获得足够短的寡糖糖结构，足以获得MS/MS的特征。最初，来自电视的馏分以MALDI-TOF MS为特征，然后使用石墨柱和Qotof质谱仪在LC-MS/MS分析之前，在LC-MS/MS进行分析之前，将寡糖部分减少并苄苄烯基化。或LTQ-Orbitrap MS系统。确定了分配给不同结构基序的各种糖。已经分配了许多结构，并进行了进一步的数据解释，并且正在进行调查。生物学表征表明，阴道脂肪磷酸t. t. t. phipophoglycan触发了人类雌性生殖道上皮细胞对细胞因子的选择性上调。最近发表了两个描述该项目结果的手稿，并在2009年德国不来梅举行的国际质谱会议上介绍了一张海报。