BIOMASS TRANSFORMATION ? CATALYSIS OF ISPG

生物质转化？

• 批准号: 8365584
• 负责人: Pinghua Liu
• 金额: $ 0.46万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365584
• 关键词: Biology; Biomass; Catalysis; Digestion; Electron Transport; Engineering; Funding; Gel; Grant; Mass Spectrum Analysis; Medicine; National Center for Research Resources; Organism; Play; Principal Investigator; Production; Proteins; Reaction; Regulation; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; United States National Institutes of Health; catalyst; cost; improved; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. IspG is a protein of 41 kD, known to play a key role as a catalyst in the engineering production of host organisms for biofuel production. According to the research results of Liu and his coworkers, IspG is present as mutli-protein-complexes. Because IspG catalyses reductive deoxygenation reactions, their partner proteins are either involved in electron transfer or related to the regulation of IspG reduction potential. To have a better understanding of the mechanism and improve the IspG activity, we have undeertaken the identification of these proteins by mass spectrometry. In-gel digestion of each of these partners followed by analysis by MALDI-TOF MS and nanoLC-MS/MS on LTQ-Orbitrap (Thermo-Fisher) are being performed to identify these partners.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 ISPG是41 kD的蛋白质，在工程生产中起着生物燃料生产的宿主生产中的催化剂作用。根据Liu和他的同事的研究结果，ISPG以Mutli-Protein-complexes的形式出现。由于ISPG催化降低的脱氧反应，其伴侣蛋白要么参与电子转移，要么与ISPG还原势的调节有关。为了更好地了解机制并改善ISPG活性，我们通过质谱法未经证实，可以鉴定这些蛋白质。在LTQ-Orbitrap上（热弯器）上进行了MALDI-TOF MS和NANOLC-MS/MS的分析，以识别这些伴侣，以识别这些合作伙伴。