CARDIAC PHENOTYPING OF ETS-1 KNOCKOUT MICE

ETS-1 敲除小鼠的心脏表型

• 批准号: 8363223
• 负责人: PAUL David GROSSFELD
• 金额: $ 0.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363223
• 关键词: 3-Dimensional; Anatomy; Cardiac; Congenital Heart Defects; Congenital chromosomal disease; Coronary artery; Disease; Embryo; Engineering; Funding; Grant; Human; Knockout Mice; Magnetic Resonance Imaging; Microscopy; Mus; National Center for Research Resources; Phenotype; Principal Investigator; Pulmonary veins; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; Ventricular Septal Defects; chromosome 11q duplication syndrome; cost; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To perform comprehensive cardiac phenotyping of ETS-1 KO mice, and compare the phenotypes to those observed in the human chromosomal disorder Jacobsen syndrome (11q Terminal Deletion Disorder) Specifically, I am very interested in collaborating with you to have you perform cardiac MRI analysis of my genetically engineered ETS-1 knockout mice. We have already shown that they have large ventricular septal defects, and I am very interested in determining, through 3-D MRI analysis, whether they might have any additional heart defects. I hope the analysis will be able to include an examination of the coronary arteries and pulmonary veins. Most of the KO mice have large ventricular septal defects (by histopathologic analysis). We want to use MRI to more comprehensively define the 3-D anatomy of these embryos, including ideally coronary artery and pulmonary vein anatomy.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 对 ETS-1 KO 小鼠进行全面的心脏表型分析，并将表型与人类染色体疾病雅各布森综合征（11q 末端缺失疾病）中观察到的表型进行比较 具体来说，我非常有兴趣与您合作，让您对我的基因工程 ETS-1 敲除小鼠进行心脏 MRI 分析。我们已经证明他们有较大的心室间隔缺损，我非常有兴趣通过 3-D MRI 分析确定他们是否可能有任何其他心脏缺陷。 我希望分析能够包括对冠状动脉和肺静脉的检查。 大多数 KO 小鼠有较大的室间隔缺损（通过组织病理学分析）。 我们希望使用 MRI 来更全面地定义这些胚胎的 3D 解剖结构，包括理想的冠状动脉和肺静脉解剖结构。