STRUCTURAL INVESTIGATIONS ON THE ETOH BMC OF E COLI, ENGINEERED ALLOSTERIC PROT

大肠杆菌 ETOH BMC 的结构研究，工程变构保护

• 批准号: 8362289
• 负责人: Martin Sagermann
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362289
• 关键词: Cholesterol Oxidase; Chromobacterium; Engineering; Escherichia coli; Ethanol; Ethanolamine Ammonia-Lyase; Ethanolamines; Funding; Grant; Investigation; Molecular; National Center for Research Resources; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resources; Solvents; Source; Structure; Time; United States National Institutes of Health; Work; beamline; cost; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Beam time is requested for three ongoing projects regarding 1) the structure and function of the bacterial ethanolamine ammonia lyase microcompartment of, E. coli 2) the engineering of allosteric functions into proteins and 3) the structure and function of the solvent-stable cholesterol oxidase from Chromobacterium sp. DS-1. The preliminary work on all research directions has resulted in crystals or crystalline material that requires high-energy x-ray sources. In all cases, we request access to monochromatic beamlines as most crystallographic structures are likely to be solved by molecular replacement. With regards to the work on the bacterial ethanolamine microcompartment, current work on the EutQ protein (see below) may require access to MAD beamlines.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 关于三个正在进行的项目，要求光束时间关于1）乙醇胺氨裂解酶微聚合物的结构和功能，大肠杆菌2）变构函数到蛋白质中的工程以及3）溶剂稳定的胆固醇氧化酶的结构和功能。 DS-1。所有研究方向上的初步工作都导致了需要高能量X射线源的晶体或结晶材料。在所有情况下，我们都要求访问单色光束线，因为大多数晶体学结构都可能通过分子置换来解决。关于在细菌乙醇胺微室的工作，当前在EUTQ蛋白上的工作（见下文）可能需要使用MAD梁线。