IMPACT OF VIRAL-MEDIATED IGF-I GENE TRANSFER ON SKELETAL MUSCLE FOLLOWING

病毒介导的 IGF-I 基因转移对骨骼肌的影响

• 批准号: 8361458
• 负责人: KRISTA H VANDENBORNE
• 金额: $ 1.22万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361458
• 关键词: Age; Anterior; Binding Proteins; Female; Funding; Gene Transfer; Grant; Hindlimb; Immobilization; Insulin-Like Growth Factor I; Insulin-Like-Growth Factor I Receptor; Mass Spectrum Analysis; Mediating; Molecular; Mus; Muscle; National Center for Research Resources; Play; Positioning Attribute; Principal Investigator; Protein Binding; Recombinant adeno-associated virus (rAAV); Recovery; Research; Research Infrastructure; Resources; Role; Skeletal Muscle; Source; United States National Institutes of Health; Viral; adeno-associated viral vector; biomedical resource; cost; muscle hypertrophy; muscle regeneration; overexpression; receptor; response; tibialis anterior muscle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Insulin-like growth factor I (IGF-I) is a potent myogenic factor that plays a critical role in muscle regeneration and muscle hypertrophy. The purpose of this study was to evaluate the effect of IGF-I overexpression on the recovery of muscle size and function during reloading/reambulation after a period of cast immobilization in predominantly fast twitch muscles. In addition, we investigated concomitant molecular responses in IGF-I receptor and binding proteins (BPs). Recombinant adeno-associated virus vector for IGF-I (rAAV-IGF-IA) was injected into the anterior compartment of one of the hindlimbs of young (3 wk) C57BL6 female mice. At 20 wk of age, both hindlimbs were cast immobilized in a shortened position for 2 wk to unload the tibialis anterior (TA) and extensor longus digitorum (EDL) muscles. The TA and EDL muscles were removed bilaterally after 2 wk of cast immobilization and after 1 and 3 wk of free cage reambulation.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 胰岛素样生长因子 I (IGF-I) 是一种有效的生肌因子，在肌肉再生和肌肉肥大中发挥着关键作用。本研究的目的是评估 IGF-I 过表达对主要是快肌群的石膏固定一段时间后重新加载/重新行走过程中肌肉大小和功能恢复的影响。此外，我们还研究了 IGF-I 受体和结合蛋白 (BP) 的伴随分子反应。将 IGF-I 的重组腺相关病毒载体 (rAAV-IGF-IA) 注射到年轻（3 周）C57BL6 雌性小鼠后肢之一的前室中。 20 周龄时，双后肢以缩短的姿势固定 2 周，以减轻胫骨前肌 (TA) 和趾长伸肌 (EDL) 的负荷。石膏固定 2 周后以及自由笼重新行走 1 周和 3 周后，去除双侧 TA 和 EDL 肌肉。