BLUETONGUE VIRUS

蓝舌病毒

• 批准号: 8361131
• 负责人: POLLY ROY
• 金额: $ 1.23万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361131
• 关键词: Caliber; Capsid; Cattle; Complex; Decontamination; Electron Microscopy; Funding; Future; Gel; Genetic Transcription; Genome; Goat; Grant; Human; Insect Vectors; National Center for Research Resources; Nucleosome Core Particle; Preparation; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Ruminants; Sampling; Sheep; Sodium Dodecyl Sulfate-PAGE; Source; Ultracentrifugation; United States National Institutes of Health; Virion; Virus; cost; macromolecule; particle; prevent

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The BTV-1 cores are double-layered (two protein layers) and are ~70nm diameter. There may be some contaminating single-layered particles which are ~52nm diameter, and these lack the VP7 protein. The transcription complexes are located inside the inner layer at the 5 fold symmetry axes (although possibly not at all 12 - the genome consists of just 10 segments). The sample appears to be 100% pure on an SDS PAGE gel and is ~0.1-0.2 mg/ml. Much higher concentrations can be achieved by ultracentrifugation of a larger preparation in the future if necessary. Storage at 4C for months is possible. The core particles (lacking the outer capsid) are much more stable than the complete virion (and easier to purify). The virus is not hazardous to humans, but decontamination with bleach is essential to prevent the potentially infectious cores from infecting the insect vector (which exists in the US) or directly coming into contact with sheep, cows, goats, or wild ruminants.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 BTV-1核是双层（两个蛋白质层），直径约为70nm。可能有一些直径约52nm的污染单层颗粒，并且缺乏VP7蛋白。转录复合物位于5倍对称轴的内层内部（尽管可能还不是所有12个 - 基因组仅由10个段组成）。 样品在SDS页面凝胶上似乎是100％纯的，〜0.1-0.2 mg/ml。如果有必要，将来可以通过更高的制备来实现更高的浓度。 可以在4C处存储几个月。核心颗粒（缺少外带帽）比完整的病毒体（并且更易于纯化）要稳定得多。 该病毒对人类没有危害，但是对漂白剂的污染对于防止潜在传染性核心感染昆虫载体（存在于美国）或直接与绵羊，牛，山羊或野性反刍动物接触至关重要。