PROCHLOROCOCCUS BACTERIA AND PHAGE INTERACTIONS

原绿球菌属细菌和噬菌体相互作用

• 批准号: 8361081
• 负责人: SALLY W CHISHOLM
• 金额: $ 4.9万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361081
• 关键词: Algae; Bacteria; Bacteriophages; Chloroplasts; Cyanobacterium; Electron Microscopy; Electrons; Funding; Grant; Infection; Marines; Microbe; National Center for Research Resources; Organism; Oxygen; Photosynthesis; Phytoplankton; Principal Investigator; Process; Prochlorococcus; Research; Research Infrastructure; Resolution; Resources; Source; Structure; Study models; United States National Institutes of Health; Work; cost; macromolecule; particle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Nearly half of Earth's atmospheric oxygen is produced by marine phytoplankton, namely phototrophic algae and cyanobacteria. Cyanobacteria, sometimes misnamed blue-green algae, are the simplest of all photosynthetic organisms and are the descendents of the ancient proto-symbiotic organisms that evolved into chloroplasts; as such, they are an excellent model for studying photosynthesis. The most numerically abundant species of these microbes is the pico-cyanobacterium, Prochlorococcus marinus. In this work, we use electron cryotomography to examine multiple strains of Prochlorococcus (MED4, MIT-9313, and Natl-1A) and the bacteriophages that infect them. In addition, we also determine the structure of the isolated phages that infect the bacteria. We aim at obtaining a snap shots of high resolution structures of the phages during the infection process. This will require a combination of electron cryotomography and single particle averaging.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 地球大气中近一半的氧气是由海洋浮游植物产生的，即 光养藻类和蓝细菌。蓝细菌，有时被错误地命名为蓝绿色 藻类是所有光合生物中最简单的，是光合生物的后代 进化成叶绿体的古代原始共生生物；因此，它们是 研究光合作用的优秀模型。 数量最丰富的物种 这些微生物是微微蓝藻、海原绿球藻。 在这项工作中，我们使用电子冷冻断层扫描技术来检查原绿球菌的多种菌株 （MED4、MIT-9313 和 Natl-1A）以及感染它们的噬菌体。此外，我们 还确定了感染细菌的分离噬菌体的结构。我们的目标是 获得感染过程中噬菌体高分辨率结构的快照 过程。这需要结合电子冷冻断层扫描和单粒子 平均。