PROCHLOROCOCCUS BACTERIA AND PHAGE INTERACTIONS

原绿球菌属细菌和噬菌体相互作用

• 批准号: 8168555
• 负责人: SALLY W CHISHOLM
• 金额: $ 8.6万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168555
• 关键词: Algae; Bacteria; Bacteriophages; Chloroplasts; Computer Retrieval of Information on Scientific Projects Database; Cyanobacterium; Electrons; Funding; Grant; Infection; Institution; Marines; Microbe; Organism; Oxygen; Photosynthesis; Phytoplankton; Process; Prochlorococcus; Research; Research Personnel; Resolution; Resources; Source; Structure; Study models; United States National Institutes of Health; Work; particle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nearly half of Earth's atmospheric oxygen is produced by marine phytoplankton, namely phototrophic algae and cyanobacteria. Cyanobacteria, sometimes misnamed blue-green algae, are the simplest of all photosynthetic organisms and are the descendents of the ancient proto-symbiotic organisms that evolved into chloroplasts; as such, they are an excellent model for studying photosynthesis. The most numerically abundant species of these microbes is the pico-cyanobacterium, Prochlorococcus marinus. In this work, we use electron cryotomography to examine multiple strains of Prochlorococcus (MED4, MIT-9313, and Natl-1A) and the bacteriophages that infect them. In addition, we also determine the structure of the isolated phages that infect the bacteria. We aim at obtaining a snap shots of high resolution structures of the phages during the infection process. This will require a combination of electron cryotomography and single particle averaging.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 地球大气中近一半的氧气是由海洋浮游植物（即光养藻类和蓝细菌）产生的。蓝细菌，有时被误称为蓝绿藻，是所有光合生物中最简单的，是进化成叶绿体的古代原始共生生物的后代。因此，它们是研究光合作用的绝佳模型。 这些微生物中数量最丰富的物种是微微蓝藻、原绿球藻。 在这项工作中，我们使用电子冷冻断层扫描技术来检查多种原绿球菌菌株（MED4、MIT-9313 和 Natl-1A）以及感染它们的噬菌体。此外，我们还确定了感染细菌的分离噬菌体的结构。我们的目标是在感染过程中获得噬菌体高分辨率结构的快照。这需要结合电子冷冻断层扫描和单粒子平均。