SINGLE PARTICLE CRYO--ET

单粒子冷冻技术

• 批准号: 8361074
• 负责人: MICHAEL F. SCHMID
• 金额: $ 12.26万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361074
• 关键词: Address; Area; Cells; Classification; Data Collection; Electron Microscopy; Electrons; Funding; Grant; Heterogeneity; Image; Molecular Conformation; National Center for Research Resources; Principal Investigator; Problem Solving; Procedures; Research; Research Infrastructure; Resolution; Resources; Source; Structure; Techniques; United States National Institutes of Health; computerized data processing; cost; experience; macromolecular assembly; macromolecule; particle; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This core project is aimed at setting up an experimental and computational procedure to address structural questions by using electron cryo-tomography (cryo-ET) followed by post- tomographic classification, alignment and averaging. Cryo-ET has emerged in recent years as a powerful technique for bridging the gap between atomistic structures and whole cells. Specifically, the recent idea to perform so called 'single particle' cryo-ET is closely related to the existing expertise at the NCMI, and we believe we can make a contribution in this area by leveraging our experience with high resolution data collection and processing. Tomography can help solve the problem of multiple conformational states posed above because of its unique ability to give three-dimensional information about a macromolecular assembly, greatly reducing the confusing interaction of structural heterogeneity with orientation that is often difficult to distinguish with a single 2D image of each particle.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该核心项目旨在设置一个实验和计算程序 通过使用电子冷冻术（Cryo-ET）解决结构性问题，然后在 层析成像分类，对齐和平均。近年来，Cryo-Et已出现 一种强大的技术，用于弥合原子结构和整个细胞之间的差距。 具体而言，最近执行所谓的“单粒子”冷冻-ET的想法与 NCMI的现有专业知识，我们相信我们可以在这一领域做出贡献 通过利用高分辨率数据收集和处理的经验。 断层扫描可以帮助解决上面提出的多种构象状态的问题 因为它具有独特的能力，可以提供有关大分子的三维信息 组装，大大减少了结构异质性与 通常很难用每个粒子的单个2D图像来区分的方向。