"SINGLE PARTICLE" CRYO-ET

“单颗粒”冷冻电离层析

• 批准号: 7953777
• 负责人: MICHAEL F. SCHMID
• 金额: $ 6.95万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953777
• 关键词: Address; Area; Cells; Classification; Computer Retrieval of Information on Scientific Projects Database; Coupling; Data Collection; Electron Microscopy; Electrons; Funding; Grant; Heterogeneity; Image; Institution; Molecular Conformation; Problem Solving; Procedures; Process; Research; Research Personnel; Resolution; Resources; Source; Structure; Techniques; United States National Institutes of Health; experience; macromolecular assembly; macromolecule; particle; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core project is aimed at setting up an experimental and computational procedure to address these structural questions by using electron cryo-tomography (cryo-ET) followed by post-tomographic classification, alignment and averaging. Cryo-ET has emerged in recent years as a powerful technique for bridging the gap between atomistic structures and whole cells [e.g. (Lucic et al., 2005)]. Specifically, the recent idea to perform so called "single particle" cryo-ET is closely related to the existing expertise at the NCMI, and we believe we can make a contribution in this area by leveraging our experience with high resolution data collection and processing. Tomography can help solve the problem of multiple conformational states posed above because of its unique ability to give three-dimensional information about a macromolecular assembly, greatly reducing the confusing coupling of structural heterogeneity with orientation that is often difficult to distinguish with a single 2D image of each particle.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该核心项目旨在建立实验和计算程序，通过使用电子冷冻术（Cryo-ET）解决这些结构性问题，然后进行体现后分类，对准和平均。近年来，Cryo-Et已成为一种强大的技术，用于弥合原子结构和整个细胞之间的差距[例如， （Lucic等，2005）]。具体而言，最近执行所谓“单粒子”的Cryo-ET的想法与NCMI的现有专业知识密切相关，我们相信我们可以通过利用高分辨率数据收集和处理的经验来为这一领域做出贡献。断层扫描可以帮助解决上面提出的多种构象状态的问题，因为它具有给出有关大分子组件的三维信息的独特能力，从而大大降低了结构异质性与方向的混乱耦合，这通常很难用每个粒子的单个2D图像来区分。