METHIONINE SULFOXIDE REDUCTASE REPAIR OF CATALASE IN HELIOBACTER PYLORI

幽门螺杆菌中过氧化氢酶的甲硫氨酸亚硫酸还原酶修复

• 批准号: 8361818
• 负责人: JOSHUA S SHARP
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361818
• 关键词: Attenuated; Bacteria; Chemicals; Enzymes; Funding; Grant; Helicobacter pylori; Hypochlorite; Incubated; Infection; Mass Spectrum Analysis; Measures; Methionine; National Center for Research Resources; Oxidants; Oxidative Stress; Oxidoreductase; Pathway interactions; Principal Investigator; Proteins; Pylorus; Research; Research Infrastructure; Resources; Side; Source; Stomach; Ulcer; United States National Institutes of Health; biological adaptation to stress; catalase; cost; methionine sulfoxide; methionine sulfoxide reductase; mutant; oxidation; repair enzyme; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The oxidative stress defenses of H. pylori are essential in allowing the bacterium to persist in its infection in the stomach, where it causes ulcers. An essential part of the bacterium's defense against oxidative stress is the methionine sulfoxide reductase repair enzyme, which can convert methionine sulfoxide to unmodified methionine. Previous studies have indicated that one of the enzymes that methionine sulfoxide reductase interacts with is catalase, another protein essential to the oxidative stress response pathways in H. pylori. In this subproject, we will oxidize catalase with a chemical oxidant such as hypochlorite, and then measure the amount of oxidation of each methionine side chain by mass spectrometry. We will then incubate the oxidized catalase with H. pylori methionine sulfoxide reductasae, including a mutant reductase with attenuated activity, and examine which methionines are repaired by the reductase.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 幽门螺杆菌的氧化应激防御对于允许细菌在胃中持续存在，在胃中持续存在，从而导致溃疡。 该细菌防御氧化应激的必不可少的部分是甲硫氨酸还原酶修复酶，该酶可以将甲硫氨酸磺代转化为未修饰的蛋氨酸。 先前的研究表明，其中一种酶是甲硫氨酸还原酶与IS Catalase相互作用，这是幽门螺杆菌中氧化应激反应途径必不可少的另一种蛋白质。 在此副标中，我们将用化学氧化剂（例如次氯酸盐）氧化过氧化氢酶，然后通过质谱法测量每个蛋氨酸侧链的氧化量。 然后，我们将将氧化过氧化酶与幽门螺杆菌硫氨酸还原酶孵育，包括带有减毒活性的突变型还原酶，并检查哪些蛋氨酸是通过还原酶修复的。