MECHANISMS OF LUMINAL ACIDIFICATION IN EPIDYDIMAS & VAS DEFERENS

附睾管腔酸化机制

• 批准号: 7953821
• 负责人: SYLVIE BRETON
• 金额: $ 0.56万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953821
• 关键词: Bicarbonates; Cell membrane; Clear Cell; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Duct (organ) structure; Electrodes; Environment; Enzymes; Epididymis; Epithelial Cells; Fertility; Funding; Goals; Grant; Institution; Intervention; Laboratories; Organ; Pathologic; Prevention; Process; Proton Pump; Proton-Translocating ATPases; Protons; Recycling; Regulation; Reproductive Physiology; Research; Research Personnel; Resources; Source; Sperm Maturation; United States National Institutes of Health; Vas deferens structure; Vesicle; apical membrane; male; premature; reproductive; reproductive function; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Active proton transport mechanisms establish and maintain a luminal acidic environment in the excurrent ducts of the male reproductive tract. The low luminal pH and a low bicarbonate concentration are important for sperm maturation, the prevention of premature activation of acrosomal enzymes, and for maintaining sperm in a quiescent state during storage in this organ. Despite this critical function in male reproductive physiology, acidification processes in the male reproductive tract are still incompletely understood. Our laboratory is studying the mechanisms that participate in luminal acidification and its regulation in the epididymis and vas deferens. The ultimate goals of this study are to understand how defective acidification may impair reproductive function in pathologic states, and how intervention to manipulate acidification may eventually be used to control male fertility. The non-invasive self-referencing proton-selective micro electrode developed in the BioCurrrents Research Center has allowed us to identify the major proton secretory mechanism in the initial part of the vas deferens. In collaboration with Peter Smith, we have shown that a bafilomycin-sensitive, vacuolar proton-pumping H+-ATPase (V-ATPase) is concentrated in the apical membrane of a subpopulation of epithelial cells (clear cells) and is a major contributor to luminal acidification. In acidifying clear cells, proton secretion is regulated via recycling of V-ATPase-containing vesicles to and from the plasma membrane. .

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 主动质子运输机制在男性生殖道的外导管中建立并维持管腔酸性环境。低腔内 pH 值和低碳酸氢盐浓度对于精子成熟、防止顶体酶过早激活以及在该器官中储存期间保持精子处于静止状态很重要。尽管在男性生殖生理学中具有这一关键功能，但男性生殖道的酸化过程仍不完全清楚。我们的实验室正在研究参与管腔酸化的机制及其在附睾和输精管中的调节。这项研究的最终目标是了解酸化缺陷如何损害病理状态下的生殖功能，以及如何通过控制酸化的干预措施最终用于控制男性生育能力。 生物电流研究中心开发的非侵入性自参考质子选择性微电极使我们能够确定输精管起始部分的主要质子分泌机制。与 Peter Smith 合作，我们发现巴弗洛霉素敏感的液泡质子泵 H+-ATP 酶 (V-ATP 酶) 集中在上皮细胞亚群（透明细胞）的顶膜中，并且是管腔的主要贡献者。酸化。在酸化透明细胞中，质子分泌是通过含有 V-ATP 酶的囊泡往返于质膜的循环来调节的。 。