University of California, San Diego Neuroscience Microscopy Imaging Core

加州大学圣地亚哥分校神经科学显微成像核心

• 批准号: 8120385
• 负责人: JOSEPH G GLEESON
• 金额: $ 34.13万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8120385
• 关键词: Address; Area; Award; California; Cells; Communities; Degenerative Disorder; Development; Disease; Dissection; Equipment; Event; Funding; Hypoxia; Image; Imaging Device; Lasers; Life; Measures; Microscopy; National Institute of Neurological Disorders and Stroke; Neurons; Neurosciences; Neurosciences Research; Phase; Productivity; Publications; Research; Research Personnel; Stroke; System; Testing; Time; Training; Universities; Work; axon guidance; flexibility; image processing; imaging modality; in vivo; injury and repair; medical schools; migration; nervous system disorder; relating to nervous system; stem cell differentiation; success; tool; two-photon

The University of California, San Diego Neuroscience Microscopy Imaging Core has grown to be a centerpiece for neuroscience research within the community, and has resulted in remarkable yields in productivity, expanded scientific scope and the ability to test hypotheses in in vivo settings. This success can be measured in over 40 publications in the neuroscience field that have critically relied on the Core to provide expertise in just the short time that it has been in existence. The Core currently has five imaging systems that provide confocal, deconvolution, spinning disk, two-photon and laser dissection microscopy and an onsite microscopist to make maximum use of the tools. The Core provides neuroscientists flexible platforms to determine the ideal imaging modality for specific applications, and provides training opportunities in a broad array of modern imaging tools. In this application, we seek to expand the scope to include several new NINDS-funded Major Users, and acquire two new imaging systems that will greatly benefit our dynamic community and address the major deficiencies of the facility: 1. Confocal imaging with the ability to perform photoinduced conversion in real- time. 2. High-throughput imaging through multi-well live cell microscopy. The requested systems were chosen because of their high image quality, unsurpassed abilities to visualize events in neural systems that were not previously possible, and their ease of use, which is critical for a facility with over 100 regular users. The research that this equipment will support includes studies of neuronal stem cell differentiation, migration, axon guidance, injury repair, stroke, hypoxia, degenerative disease and disordered development that has important implications for understanding and treatment of nervous system disease. The Core draws off of the expertise of the larger UCSD Medical School Imaging Core that combines other complementary tools. The flexibility, dynamic range, sensitivity and image processing capabilities with the proposed tools are essential for the next phase of the NINDS-funded work.

加利福尼亚大学圣地亚哥神经科学显微镜成像核心已成为社区内神经科学研究的核心，并导致生产力的产量显着，扩大了科学范围以及在体内环境中测试假设的能力。可以在神经科学领域的40多个出版物中衡量这一成功，这些出版物在仅在短时间内就依靠核心来提供专业知识。核心目前具有五个成像系统，可提供共焦，反向卷积，旋转磁盘，两光子和激光解剖显微镜以及一个现场显微镜，以最大程度地利用工具。核心提供神经科学家灵活的平台，以确定特定应用程序的理想成像方式，并在广泛的现代成像工具中提供培训机会。 在此应用程序中，我们试图扩大范围，以包括几个新的Ninds资助的主要用户，并获得了两个新的成像系统，这些系统将极大地使我们的动态社区受益，并解决该设施的主要缺陷：1。共聚焦成像，具有实时执行照片诱导的转换的能力。 2。通过多孔活细胞显微镜进行高通量成像。之所以选择所请求的系统，是因为它们的图像质量很高，无法可视化以前无法使用的神经系统中的事件，并且它们的易用性，这对于拥有100多名常规用户的设施至关重要。该设备将支持的研究包括对神经元干细胞分化，迁移，轴突指导，损伤修复，中风，缺氧，退行性疾病和无序发育的研究，对理解和治疗神经系统疾病具有重要意义。核心借鉴了更大的UCSD医学院成像核心的专业知识，该核心结合了其他互补工具。提出的工具的灵活性，动态范围，灵敏度和图像处理能力对于Ninds资助的工作的下一阶段至关重要。