X-RAY DATA COLLECTION OF PROTEINS INVOLVED IN CELL ADHESION

细胞粘附相关蛋白质的 X 射线数据收集

• 批准号: 7954557
• 负责人: TINA IZARD
• 金额: $ 0.1万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954557
• 关键词: Actinin; Actins; Adherens Junction; Adhesions; Binding; Cadherins; Cell Adhesion; Cells; Coin; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytoskeleton; Data Collection; Event; Extracellular Matrix; Focal Adhesions; Funding; Grant; Growth and Development function; Institution; Integrins; Link; Mediating; Molecular; Morphogenesis; Myopathy; Neoplasm Metastasis; Play; Process; Proteins; Research; Research Personnel; Resources; Roentgen Rays; Source; Structure; Talin; United States National Institutes of Health; Vinculin; cell motility; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell migration and morphogenesis are essential for the development, growth, and survival of metazoans, and these processes are also at play in pathophysiological scenarios such as cancer metastasis and myopathies. These events rely on the cell?s ability to dynamically form and break specific contacts, coined adhesion junctions, with its neighbors (adherens junctions) or the extracellular matrix (focal adhesions). Vinculin is an essential regulator of both cell-cell (cadherin-catenin mediated) and cell-matrix (integrin-talin mediated) junctions, where it provides links to the actin cytoskeleton by binding to talin in integrin complexes, or to -catenin and -actinin in cadherin junctions. To understand the molecular details of the dynamics in adhesion complexes, we will solve the crystal structures of several proteins involved in cell adhesion in complex with their binding partners.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 细胞迁移和形态发生对于后生动物的发育、生长和生存至关重要，这些过程也在癌症转移和肌病等病理生理情况中发挥作用。这些事件依赖于细胞与其邻居（粘附连接）或细胞外基质（粘着斑）动态形成和破坏特定接触、创造的粘附连接的能力。纽蛋白是细胞-细胞（钙粘蛋白-连环蛋白介导）和细胞-基质（整联蛋白-踝蛋白介导）连接的重要调节剂，通过与整联蛋白复合物中的踝蛋白或-连环蛋白和-结合，提供与肌动蛋白细胞骨架的连接。钙粘蛋白连接处的肌动蛋白。为了了解粘附复合物动力学的分子细节，我们将解析复合物中涉及细胞粘附的几种蛋白质及其结合伙伴的晶体结构。