MOUSE BRAIN NEUROANATOMICAL PHENOTYPING

小鼠大脑神经解剖表型分析

• 批准号: 8363143
• 负责人: ALEXANDRA BADEA
• 金额: $ 2.45万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363143
• 关键词: Behavior; Blood Vessels; Brain; Cerebellum; Corpus striatum structure; Data; Funding; Gene Expression; Genetic; Grant; Hippocampus (Brain); Histology; Image; Individual; Microscopy; Mus; National Center for Research Resources; Phenotype; Principal Investigator; Protocols documentation; Research; Research Infrastructure; Resolution; Resources; Source; Staining method; Stains; United States National Institutes of Health; Variant; cost; non-genetic; trait

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Use MR histology to obtain data on natural variation of neuroanatomical and CNS vascular phenotypes in the mouse brain to study in relation to known differences in brain gene expression and behavior. MR histology with its high quality images of stained mouse brains and resolution of 20 ¿m can provide accurate quantitative data on the neuroanatomical and vascular traits. The intra- and inter-strain comparisons of morphometric parameters will provide estimates of both genetic and nongenetic variability among these strains (n = 12). The imaging protocols would allow identification of 21 individual neuroanatomical stuctures (i.e hippocampus, striatum, cerebellum, etc.) on which morphometric analysis can be performed. These parameters would be correlated with several hundred thousand other parameters already quantified in these same strains.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 使用MR组织学获取有关小鼠大脑中神经解剖学和CNS血管表型自然变异的数据，以研究与脑基因表达和行为的已知差异有关。 MR组织学及其高质量的小鼠大脑图像和20€的分辨率可以提供有关神经解剖学和血管性状的准确定量数据。形态计量参数的内部和晶体间比较将提供这些菌株中遗传变异性和非遗传变异性的估计（n = 12）。成像方案将允许鉴定21种单个神经解剖结构（即海马，纹状体，小脑等），可以在其中进行形态计量分析。这些参数将与已经在这些相同菌株中量化的数十万个参数相关。