COBRE: U KS: CORE B: PROTEIN PURIFICATION GROUP

COBRE：UK：Core B：蛋白质纯化组

• 批准号: 8359658
• 负责人: Fei Philip Gao
• 金额: $ 18.57万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359658
• 关键词: Area; Binding; Cells; Centers of Research Excellence; Cloning; Equipment; Fermentation; Funding; Generations; Grant; Housing; Human Resources; Incubators; Individual; Insecta; Kansas; Laboratories; Mutagenesis; National Center for Research Resources; Plasma; Principal Investigator; Production; Property; Protein Analysis; Proteins; Research; Research Infrastructure; Research Personnel; Resources; Services; Source; Surface; System; Systems Development; Time; Training; United States National Institutes of Health; Universities; Work; cost; expression cloning; fast protein liquid chromatography; high throughput screening; overexpression; protein B; protein expression; protein purification; protein structure; protein structure function; protocol development; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The COBRE Core B Protein Production Core Laboratory is hosted within The University of Kansas Higuchi Bioscience Center and is housed in the Shankel Structural Biology Center. The lab focuses on the cloning, expression and purification of prokaryotic and eukaryotic proteins for researchers who need to obtain proteins for their research, which adds technical support to individual research groups. The Core works closely with the Bio-NMR Core Laboratory, the Protein Structure Core Laboratory, and the High Throughput Screening Laboratory. The Core B laboratory provides a one-stop menu of services to investigators, including protocol development; cloning; mutagenesis; expression system development or improvement; large or small scale protein expression and/or purification; Biacore binding studies; and technical training for researchers in any of these areas. The staff can perform protein expression in either bacterial or insect cell systems. The core laboratory possesses the equipment and personnel required for overexpression and purification of properly folded and functional proteins in quantities sufficient for a variety of applications, including functional and binding studies. The major equipment in the facility includes: Shaker-incubators; Automated fermentation (1-10 L scale); AKTAXpress, AKTA Purifier system and FPLC system; Constant Systems Hydraulic Cell Disruptor, and a Biacore 3000 Surface Plasma Resonance system. In addition, the laboratory provides advice on the generation and analysis of proteins depending on their physicochemical properties and the needs of the investigator. The staff consists of a Director and one full time technician.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 COBRE B蛋白生产核心实验室主持在堪萨斯大学Higuchi Bioscience Center内，并位于Shankel结构生物学中心。该实验室的重点是针对需要为其研究获得蛋白质的研究人员的原核生物和真核蛋白的克隆，表达和纯化，这为单个研究小组增加了技术支持。核心与Bio-NMR核心实验室，蛋白质结构核心实验室和高吞吐量筛查实验室紧密合作。核心B实验室为调查人员提供了一站式服务，包括协议开发；克隆;诱变；表达系统的发展或改进；大小的蛋白质表达和/或纯化； BIACORE结合研究；以及在任何这些领域的研究人员的技术培训。工作人员可以在细菌或昆虫细胞系统中执行蛋白质表达。核心实验室拥有过度表达和纯化适当折叠和功能性蛋白质所需的设备和人员，其数量足以用于各种应用，包括功能和结合研究。该设施中的主要设备包括：振荡器启动器；自动发酵（1-10升比例）； Aktaxpress，Akta净化器系统和FPLC系统；恒定系统液压细胞破坏器和BIACORE 3000表面等离子体共振系统。此外，实验室根据其物理化学特性和研究者的需求提供有关蛋白质生成和分析的建议。员工由董事和一名全职技术人员组成。