GENOMICS CORE

基因组核心

基本信息

批准号：
8360188
负责人：
Donald Anthony Primerano
金额：
$ 14.39万
依托单位：
MARSHALL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-05-01 至 2012-04-30
项目状态：
已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Microarray experimentation and automated DNA sequencing require sophisticated methods, expensive instrumentation and reagents, and intense data management and analysis. The Genomics Core will provide centralized services that would not be available to smaller institutions and provide training in methods needed for completion of biomedical research projects. Our primary objective is to enable the genomic research goals of INBRE, COBRE and other research projects at WV universities and colleges. Under WV-INBRE Phase II the Genomics Core Facility will provide the following services: (1) microarray-based gene expression profiling and statistical support for data analysis (2) automated DNA sequencing and access to DNA sequence analysis software in collaboration with the WV-INBRE Bioinformatics Core, (3) access to real-time thermal cyclers for quantitative PCR and to an Agilent 2100 Bioanalyzer for DNA/RNA quantitation and quality assessment, and (4) purification and banking of patient genomic DNA. We will also provide workshops in conjunction with the Bioinformatics Core on methods central to research plans of the undergraduate research projects. All core services will be available at the start of the grant period. There is substantial need for these services among INBRE, COBRE and other investigators. It is our expectation that the Genomics Core Facility will enable INBRE and COBRE investigators to identify and characterize candidate genes involved in cancer and cardiovascular disease, provide direct evidence for experimental hypotheses, confirm patterns of gene expression, and gain insights into the physiological and biochemical functions of the candidate genes. Personnel Title D. Primerano, PhD Director J. Denvir, PhD Genomic Data Analyst G. Boskovic, PhD Microarray Manager Y. Dementieva, PhD Biostatistician Liping Wei, MS Genomics Core Manager Jun Fan, PhD Genomics Core Manager (July 1 to present) Ivana Yang, PhD Bioinformatics consultant Mark Flood, PhD Pyrosequencing Core @FSU

该副本是利用资源的众多研究子项目之一由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持而且，副投影的主要研究员可能是其他来源提供的包括其他NIH来源。列出的总费用可能代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。微阵列实验和自动DNA测序需要复杂的方法，昂贵的仪器和试剂以及强烈的数据管理和分析。基因组学核心将提供集中的服务，这些服务将无法为较小的机构提供，并提供完成生物医学研究项目所需的方法的培训。我们的主要目标是实现WV大学和大学INBRE，COBRE和其他研究项目的基因组研究目标。在WV-II阶段，基因组学核心设施将提供以下服务：（1）基于微阵列的基因表达分析和数据分析的统计支持（2）与WV-INBRE生物信息学核心合作的自动DNA测序和访问DNA序列分析软件，（3）访问定量PCR的实时热循环器和Agilent 2100生物分析仪进行DNA/RNA定量和质量评估，以及（4）患者基因组DNA的纯化和库存。我们还将提供与本科研究项目研究计划中心的生物信息学核心结合的研讨会。所有核心服务将在赠款期开始时提供。在Inbre，Cobre和其他调查人员中，这些服务非常需要这些服务。我们期望基因组学核心设施能够使近野和毛cre研究者能够识别和表征参与癌症和心血管疾病的候选基因，为实验假设提供直接证据，确认基因表达模式，并获得对生理和生物化学功能的见解。候选基因。人事头衔 D. Primerano，博士主任 J. Denvir，博士基因组数据分析师 G. Boskovic，博士微阵列经理 Y. Dementieva，博士生物统计学家 MS Genomics Core Manager MS Liping Wei Jun Fan，PhD Genomics Core Manager（7月1日出现）伊万娜·杨（Ivana Yang），博士生物信息学顾问 Mark Flood，PhD pyrosequencing Core @fsu