MARSHALL U COBRE: GENOMICS COREP=C

马歇尔·U·科布雷：基因组学 COREP=C

• 批准号: 7720782
• 负责人: Donald Anthony Primerano
• 金额: $ 38.94万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720782
• 关键词: Area; Candidate Disease Gene; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; DNA Sequence; DNA Sequence Analysis; Data; Data Analyses; Development; Experimental Designs; Funding; Gene Expression; Gene Expression Profiling; Genomics; Goals; Grant; Human Resources; Institution; Investigation; Malignant Neoplasms; Marshal; Measurement; Messenger RNA; Methods; Molecular; Pathway interactions; Polymerase Chain Reaction; Positioning Attribute; Reagent; Research; Research Personnel; Research Project Grants; Resources; Sequence Analysis; Signal Transduction; Source; Time; Training; United States National Institutes of Health; Universities; base; data management; expectation; experience; insight; instrumentation; novel; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Each research project within this COBRE application relies heavily on gene expression analysis for the completion of their specific aims. The most common genomic approaches in this application are gene expression profiling by microarray, single mRNA measurement abundance by real-time PCR, and DNA sequencing and sequence analysis. Microarray experimentation and DNA sequencing require expensive instrumentation and reagents, sophisticated methods, and intense data management and analysis. These tasks are most efficiently executed by core facility personnel. The proposed COBRE projects cannot be completed without direct access to expert genomic technical and analytical support. Therefore, the objective of this application is to implement a Genomic Core Facility that will serve the specific needs of the COBRE and Marshall University investigations in the areas of microarray-based gene expression profiling, real-time PCR, automated DNA sequencing and DNA sequence analysis. Both the microarray manager and genomic data analyst will require substantial external training to keep pace with developments and provide useful analyses for the end users. The long-term goal of the Genomics Core Facility is to position COBRE investigators to discover novel molecular pathways in cancer development. We will enable the genomic research goals of the COBRE research projects by pursuing the following two specific aims: 1. Implement a Genomics Core Facility and develop Microarray Data Analysis capabilities. The Genomic C o re Facility will be co-directed by Drs Niles and Primerano who have complementary skills in experimental design and core management. Given the experience of the co-directors and presence of key personnel and instrumentation, we are well prepared to develop the Genomics Core Facility. It is our expectation that the Genomics Core Facility will enable COBRE and Marshall University investigators to identify candidate genes that are critically involved in cell signal transduction, confirm pattems of expression, and gain insights into cancer molecular biology.t?doF¿

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中央赠款提供的资源 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，在列出的机构中可能会被压制 对于中心，这不一定是调查员的工厂。 该眼镜蛇应用程序中的每个研究项目都在很大程度上取决于基因表达 分析其特定目标。 该应用中的方法是通过微阵列，单个mRNA进行基因表达分析 实时PCR的测量丰度以及DNA测序和序列分析。 微阵列实验和DNA测序昂贵的仪器和 试剂，复杂的方法以及强烈的数据管理和分析 最有效地由核心设施人员执行 直接访问专家基因组技术和分析，无法完成 因此，该应用的目的是实施基因组核心 将满足山cor和马歇尔大学的特定需求的设施 在基于微阵列的基因表达分析的领域进行了研究，实时PCR， 自动DNA测序和DNA序列分析。 以及具有大量外部培训的基因组数据分析师 开发并为最终用户提供有用的分析。 基因组学核心设施是将毛毛调查员定位以发现新的分子 癌症发展的途径。 通过追求以下两个特定AMS：1。 基因组学核心设施和开发微阵列数据分析能力 具有基因组的设施将由具有 鉴于经验，实验设计和核心管理的互补技能 在关键人员和仪器的共同导演和存在中，我们很好 准备开发基因组学核心设施。 核心设施Wille Cobre和Marshall通用调查人员确定 参与细胞信号转导至关重要的候选基因，确认 表达，并洞悉癌症分子生物学。