COBRE: UNE MED CTR: CORE A: ADMINISTRATIVE

COBRE：UNE MED CTR：核心 A：行政

基本信息

批准号：
8360390
负责人：
Shelley D Smith
金额：
$ 38.59万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2012-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8360390
关键词：
Address Advisory Committees Animal Model Blindness Cells Centers of Research Excellence Collaborations Communication Core Facility Development Education Ensure Esthesia Evaluation Faculty Funding Gene Expression Gene Mutation Genetic Genome engineering Goals Grant Hearing Hearing Impaired Persons Histology Institution Intervention Labyrinth Maintenance Mentors Mitotic Molecular Molecular Biology National Center for Research Resources Natural regeneration Nebraska Occupations Participant Pathology Pathway interactions Phase Pluripotent Stem Cells Principal Investigator Quality of life Regenerative Medicine Research Research Activity Research Infrastructure Research Personnel Resources Retina Sensory Source Syndrome System United States National Institutes of Health Vision cell injury cost design interdisciplinary approach member molecular phenotype mouse genome mouse model neurosurgery operation reconstitution retinal progenitor cell stem cell population

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The loss of neurosensory function, particularly the sensations of hearing and vision, has devastating effects on communication, education, occupation, and quality of life. Since neurosensory cells of the inner ear and retina are not replaced after damage or degeneration, such losses are permanent. The purpose of the Nebraska Center for the Molecular Biology of Neurosensory Systems is to characterize the genetic mechanisms controlling the development and maintenance of neurosensory functions and the corresponding pathology associated with relevant gene mutations to identify potential avenues for intervention. We will focus our efforts on promising regenerative medicine strategies such as the reconstitution of damaged cells through the use of pluripotent stem cells or de-differentiation and regeneration of post-mitotic cells in the inner ear and retina. These studies utilize specialized animal models that allow control of gene expression at specific critical points in development, with detailed evaluation of the associated findings at the molecular and phenotypic levels. To facilitate these studies, 3 independent research institutions have joined to build an interactive Center with a multidisciplinary approach designed to address to these well-defined objectives. With a core of 3 senior researchers and an exemplary External Advisory Committee, we have successfully mentored 14 junior faculty members and created a collaborative group united by similar thematic and mechanistic approaches. Our research has been facilitated by 3 scientific cores: a Mouse Genome Engineering core for the development of mouse models, a Microarray core for comprehensive assessment of gene expression levels and determination of possible genetic networks, and a Molecular Phenotyping/Histology core to study the results of experimental alterations. These cores are an important addition to the research infrastructure of all 3 institutions. In the second phase of this COBRE project, we will continue to support 6 junior researchers: 2 are studying the mechanisms of specific syndromes of blindness and deaf/blindness, 1 is concentrating on the development and enrichment of retinal stem cell populations, and 3 are studying the molecular pathways of inner ear differentiation with the goal of facilitating sensory regeneration. As those projects obtain external funding and rotate off Center funding, new projects will be added. The addition of new investigators, along with continued support of research activities and the operation of core facilities, will ensure a growing "critical mass" of collaboration that will sustain the Center, and enable participants to obtain external funding which will ensure its long term viability.

该副本是利用资源的众多研究子项目之一由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持而且，副投影的主要研究员可能是其他来源提供的包括其他NIH来源。列出的总费用可能代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。神经感觉功能的丧失，尤其是听力和视力的感觉，对沟通，教育，职业和生活质量产生了破坏性的影响。由于损坏或变性后，内耳和视网膜的神经感觉细胞未替换，因此这种损失是永久性的。内布拉斯加州神经感觉系统分子生物学中心的目的是表征控制神经感觉功能发展和维持的遗传机制，以及与相关基因突变相关的相应病理，以识别潜在的干预途径。我们将重点放在有希望的再生医学策略上，例如通过使用多能干细胞或内耳和视网膜中有丝分裂后细胞的脱不同和再生来重建受损细胞。这些研究利用了专门的动物模型，可以控制发育中特定临界点的基因表达，并详细评估了分子和表型水平的相关发现。为了促进这些研究，3家独立的研究机构加入了一个互动中心，采用多学科方法来解决这些定义明确的目标。我们拥有3位高级研究人员和一个模范外部咨询委员会的核心，我们成功地指导了14名初级教职员工，并通过类似的主题和机械方法建立了一个合作集团。 3个科学核心促进了我们的研究：用于开发小鼠模型的小鼠基因组工程核心，用于全面评估基因表达水平和可能的遗传网络的微阵列核心，以及分子表型/组织学核心，以研究实验变化的结果。这些核心是所有三个机构研究基础设施的重要补充。在该毛病项目的第二阶段中，我们将继续支持6个初级研究人员：2个正在研究盲目和聋/失明的特定综合症的机制，1集中于视网膜干细胞群体的发展和富集，3个正在研究内耳分子的分子途径，内部耳朵的分子途径与便利感官的促进目标。当这些项目获得外部资金并旋转中心资金时，将添加新项目。新的研究人员的增加，以及持续支持研究活动和核心设施的运作，将确保越来越多的“临界”协作质量来维持中心，并使参与者能够获得外部资金，以确保其长期的生存能力。