PREVENTION OF ORAL CANCER BY TEA

通过茶预防口腔癌

• 批准号: 7951989
• 负责人: FUNG-LUNG CHUNG
• 金额: $ 48.05万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7951989
• 关键词: Apoptosis; Area; Biological Assay; Black Tea; Blood; Cells; Cigarette; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA analysis; Deglutition; Double-Blind Method; Enzymes; Funding; Grant; Green tea; High Pressure Liquid Chromatography; Institution; Molecular; Oral; Placebos; Pleomorphism; Powder dose form; Protocols documentation; RNA; Randomized Controlled Clinical Trials; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Running; Smoker; Source; Tea; United States National Institutes of Health; Urine; Water; drinking; experience; keratinocyte; non-smoker; oral cancer prevention; polyphenol; prospective; protein expression; treatment duration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is R01-funded double-blind, prospective, randomized trial assessing the molecular and cellular effects of tea treatment on oral cells in smokers (> 20 cigarettes/day) and non smokers. The treatments are: green tea (400 mg of tea powder per cup), black tea, caffeinated water, and water (placebo). There is a 3-week run in period, a 4-week treatment period, and a 2-week washout period. The subjects will drink and swallow the materials in a prescribed specific manner; a total of 5 cups/day will be consumed. The PI is extremely experienced in this area. The cellular studies to be performed are varied. Oral keratinocytes will be examined for: pleomorphism, hyperchromatism, binucleation, analysis of DNA, enals and GSH, RT-PCR for enzyme expression, and protein and RNA isolation. Urine will be studied by HPLC for tea polyphenols and blood DNA will be analyzed. Apoptosis will be studied. Page 12 of the protocol indicates the studies to be performed but it is extremely cursory without a specific description of each assay, it's use, and possible interferences or problems.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这是 R01 资助的双盲、前瞻性、随机试验，评估茶疗法对吸烟者（> 20 支/天）和非吸烟者口腔细胞的分子和细胞影响。治疗方法包括：绿茶（每杯 400 毫克茶粉）、红茶、含咖啡因的水和水（安慰剂）。有 3 周的磨合期、4 周的治疗期和 2 周的冲洗期。受试者按照规定的特定方式饮用和吞咽材料；每天总共消耗 5 杯。 PI 在这方面非常有经验。要进行的细胞研究是多种多样的。将检查口腔角质形成细胞：多形性、深染性、双核、DNA、烯醛和谷胱甘肽分析、酶表达的 RT-PCR 以及蛋白质和 RNA 分离。将通过 HPLC 研究尿液中的茶多酚，并分析血液 DNA。将研究细胞凋亡。方案第 12 页指出了要进行的研究，但非常粗略，没有对每个测定、其用途以及可能的干扰或问题进行具体描述。