Epigenomics Core

表观基因组学核心

• 批准号: 8288817
• 负责人: John Greally
• 金额: $ 2.44万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8288817
• 关键词: Adipocytes; Administrator; Area; Beta Cell; Bioinformatics; Biological; Biological Assay; Cells; Chromatin; Commit; Complications of Diabetes Mellitus; Computational Biology; Core Facility; DNA Methylation; Data Analyses; Data Set; Data Storage and Retrieval; Development; Diabetes Mellitus; Educational workshop; Fostering; Gene Expression; Generations; Genetic; Goals; Hepatocyte; High Performance Computing; Human; Immune; Informatics; Instruction; Insulin; Journals; Kidney; Laboratories; Metabolic Diseases; Metabolism; Methodology; Mission; Modification; Molecular; Molecular Genetics; Mutation; Neurons; Obesity; Patients; Population; Postdoctoral Fellow; Process; Protocols documentation; Quality Control; Research; Research Personnel; Research Training; Resources; Rodent Model; Role; Scientist; Services; Skeletal Muscle; Students; System; Tissues; Training; Vascular Endothelial Cell; Work; blood glucose regulation; candidate identification; cohort; comparative; computing resources; cost effective; diabetes mellitus genetics; epigenomics; high throughput screening; improved; mouse model; quality assurance; relational database; tool

The mission of the Epigenomics Core is to assist investigators in performing specialized epigenomics assays and comprehensive data analyses of these large datasets. Through collaborative efforts with the other Cores of the DRTC, the effects of defined pharmacological, dietary, environmental and genetic alterations are thoroughly characterized for their effects on glucose homeostasis, insulin action, and metabolism. The role of genetic modifications in relevant tissues, such as neurons, hepatocytes, skeletal muscle, adipocytes, beta and immune cells that are related to glucose homeostasis can be directly compared by thorough and definitive experimentation in rodent models. Identification of candidate targets can be functionally confirmed or refuted by the generation of specific mouse models and validated in human populations by comparative analyses in multiple cohorts/populations. Although this is a rapidly developing field, the broad expertise assembled at Einstein's Center for Epigenomics including molecular genetics, bioinformatics and computational biology expertise allows the Epigenomics Core to provide services to DRTC investigators with a wide range of specialized, high quality methodologies and tools relevant to understanding metabolic disease processes. To accomplish these goals, the Epigenomics Core will: 1) make available to investigators specialized high-throughput molecular technological resources including microarray and massively-parallel sequencing platforms to study DNA methylation and chromatin organization; 2) provide extensive quality controls and assurance information for all high-throughput assays, generated as part of each analysis; 3) provide readily available dedicated systems administrators and programmers, relational database services, high-performance computing resources and data storage/backup systems through the Computational and Statistical Epigenomics Resource and Research Informatics; 4) advise investigators regarding the best experimental platforms and protocols to use for the specific biological question being asked; 5) disseminate current and new technological development information and foster investigator Interactions through weekly workshops and journal clubs; and 6) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing epigenomics methodologies and data analyses. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

表观基因组学核心的使命是协助研究人员进行专门的表观基因组学分析 并对这些大型数据集进行全面的数据分析。通过与其他核心的协作努力 根据 DRTC，定义的药理、饮食、环境和遗传改变的影响是 对其对葡萄糖稳态、胰岛素作用和代谢的影响进行了彻底的表征。的作用 相关组织的基因修饰，如神经元、肝细胞、骨骼肌、脂肪细胞、β 与葡萄糖稳态相关的免疫细胞可以通过彻底和全面的直接比较 啮齿动物模型的确定性实验。可以对候选目标的识别进行功能确认 或者通过生成特定小鼠模型来驳斥并通过比较在人群中进行验证 对多个队列/人群进行分析。尽管这是一个快速发展的领域，但广泛的专业知识 聚集在爱因斯坦表观基因组学中心，包括分子遗传学、生物信息学和 计算生物学专业知识使 Epigenomics Core 能够为 DRTC 研究人员提供服务 与理解代谢相关的各种专业、高质量的方法和工具 疾病过程。为了实现这些目标，表观基因组学核心将：1) 向研究人员提供 专业的高通量分子技术资源，包括微阵列和大规模并行 研究 DNA 甲基化和染色质组织的测序平台； 2）提供广泛的质量 作为每次分析的一部分生成的所有高通量测定的控制和保证信息； 3） 提供随时可用的专用系统管理员和程序员、关系数据库服务、 通过计算和统计表观基因组学资源和研究信息学提供高性能计算资源和数据存储/备份系统； 4) 就针对所提出的特定生物学问题使用的最佳实验平台和方案向研究人员提供建议； 5）通过每周研讨会和期刊俱乐部传播当前和新技术发展信息并促进研究者互动； 6) 为学生、博士后研究员、研究人员和技术人员提供表观基因组学方法和数据分析的实验室培训。所有这些服务都可供刚接触糖尿病研究的研究人员以及从事糖尿病相关项目的研究人员使用，这些项目可以通过使用该核心的专业知识和设施来丰富和扩展。