DEVELOPMENT OF A BABOON MODEL OF LIVER CANCER

狒狒肝癌模型的研制

• 批准号: 8357717
• 负责人: Robert E LANFORD
• 金额: $ 3.56万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357717
• 关键词: Animal Model; Autologous; Cancer Etiology; Cell Transplants; Cells; Cessation of life; Development; Diagnosis; Diagnostic; Funding; Gene Transduction Agent; Gene-Modified; Genes; Genetic; Grant; Hepatocyte; Imaging Techniques; Lentivirus Vector; Liver; Malignant Neoplasms; Malignant neoplasm of liver; Methods; Modeling; Modification; National Center for Research Resources; Oncogenes; Papio; Primates; Principal Investigator; Property; Research; Research Infrastructure; Resources; SCID Mice; Source; Staging; System; United States National Institutes of Health; anticancer research; cancer therapy; cost; gene cloning; mouse model; new technology; programs; tumor; tumorigenic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This is a pilot program to create a new model for liver cancer research and therapy. We will create a baboon model of liver cancer for use in new technologies involving advanced diagnostics and therapies using sophisticated imaging techniques to visualize and treat the tumor. Currently no large animal model of liver cancer is available to develop and perfect these methods. Liver cancer is the 4th leading cause of cancer death worldwide. Because it is a fast-growing tumor that is rarely diagnosed in the early stages, liver cancer is typically fatal within a year of diagnosis. In this program we will clone the genes involved in liver cancer from the baboon, such that all genes involved are homologous to the host. We will introduce genetic modifications to convert the genes into cancer inducing genes or oncogenes. We will use gene therapy vectors (Lentivirus vectors) to introduce multiple modified genes into liver cells (hepatocytes) growing in our well established hepatocyte culture system for baboon hepatocytes. Cells with tumorigenic properties will first be selected in the SCID mouse model and then introduced into the liver of baboon that was the original donor of the cells (autologous cell transplant). Once perfected, the model will be used to advance new therapies for liver cancer.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 这是一个旨在创建肝癌研究和治疗新模式的试点项目。我们将创建一个狒狒肝癌模型，用于涉及先进诊断和治疗的新技术，使用复杂的成像技术来可视化和治疗肿瘤。目前还没有大型肝癌动物模型可用于开发和完善这些方法。肝癌是全球第四大癌症死亡原因。由于肝癌是一种生长快速的肿瘤，在早期阶段很少被诊断出来，因此肝癌通常在诊断后一年内致命。在这个项目中，我们将从狒狒身上克隆与肝癌有关的基因，使所有涉及的基因与宿主同源。我们将引入基因修饰，将基因转化为癌症诱导基因或癌基因。我们将使用基因治疗载体（慢病毒载体）将多种修饰基因引入在我们完善的狒狒肝细胞肝细胞培养系统中生长的肝细胞（肝细胞）中。首先在 SCID 小鼠模型中选择具有致瘤特性的细胞，然后将其引入作为细胞原始供体的狒狒肝脏中（自体细胞移植）。一旦完善，该模型将用于推进肝癌的新疗法。