ANALYTICAL AND RESOURCE CORE SERVICES

分析和资源核心服务

• 批准号: 8357291
• 负责人: JOHN P CAPITANIO
• 金额: $ 4.79万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357291
• 关键词: Allergens; Antibodies; Antigens; Area; Bioinformatics; Biological Assay; Blood; Blood specimen; Bronchoalveolar Lavage; Buffers; Businesses; California; Candidate Disease Gene; Cell Separation; Cells; Clinical; Clinical Research; Color; Communication; Communities; Consultations; Custom; DNA; Detection; Diagnostic; Endocrine; Epithelial Cells; Evaluation; Experimental Designs; Fibroblasts; Flow Cytometry; Funding; Gene Targeting; Genetic; Genome; Grant; HIV; Health Services Accessibility; Health Status; Histamine; Hormones; IgE; Image; Immunoglobulin G; Immunohistochemistry; Immunology; Immunomodulators; In Vitro; Individual; Infectious Agent; Infectious Disease Immunology; Laboratories; Lasers; Life; Link; Lung; Lung diseases; Lymphoid Tissue; Macaca mulatta; Microspheres; Mitochondrial DNA; Modeling; Molecular Analysis; Monitor; Mucous Membrane; National Center for Research Resources; Organization administrative structures; Pathogen detection; Plasma; Preparation; Primates; Principal Investigator; Process; Proteins; RNA; Reagent; Research; Research Infrastructure; Research Personnel; Research Support; Resources; SIV; Sampling; Scientist; Services; Simian virus 40; Site; Source; Staining method; Stains; Steroids; Structure; Structure of parenchyma of lung; Surrogate Markers; Technical Expertise; Testing; Tissues; Training; United States National Institutes of Health; Viral; animal colony; assay development; cell type; cost; human tissue; instrument; interest; monoamine; nonhuman primate; pathogen; tissue processing; viral DNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Analytical and Resource Core was established in January, 2008 to 1) increase communication and efficiencies among laboratories all of which provide, in one form or another, assay services; 2) provide a larger organizational unit that could buffer fluctuations in the amount of business conducted by each individual laboratory; and 3) facilitate user access to the services, and especially to the resources, offered by CNPRC. The "Analytical" component of the ARC includes service functions associated with previously independent Cores at CNPRC, and the "Resource" component of this Core makes available viral stocks, reagents, tissues, and other resources available at CNPRC. Because many of these resources were developed by the same laboratories that provide assay services, the two functions, services and resources, were combined into a single Core. The structure of the ARC comprises the following seven CNPRC laboratories: Clinical Laboratory provides clinical and research support to internal CNPRC units, as well as to outside investigators using our facility, for monitoring the health status of colony animals for both routine and experimental purposes Endocrine Core Laboratory provides to investigators a service of hormone analysis as well as consultation relating to endocrine research conducted with nonhuman primates. This Core Laboratory also develops and validates steroid, monoamine, and protein hormone assays as needed by investigators. Flow Cytometry Core Laboratory provides access to, and services utilizing, two instruments, a FACS Calibur, which can be used for standard 4-color assays, and a FACS Aria, which has 3 lasers and can be used for multicolor flow cytometric analysis and for live cell sorting. The Laboratory performs the staining, acquisition, and analysis of standard Clinical Laboratory panels, but Principal Investigators can request custom panels. Genetics Core Laboratory provides research support in the following areas: custom DNA extraction and purification, whole genome amplification, SNP, STR and mtDNA assays; bioinformatic analysis including whole genome and target region linkage and association analyses; and candidate gene evaluation. Infectious Disease Immunology Core Laboratory provides the following services: blood and tissue processing (including mucosal tissues), antibody ELISAs against whole SIV, flow cytometric assays for phenotypic and functional characterization of multiple cell types (through the Flow Cytometry Core Laboratory), RNA / DNA isolation (blood and tissues) and analysis for several target genes. In addition, the Luminex multiplex microbead assay for the detection of antibodies and multiple immunomodulators in plasma or culture supernatants is available; this includes use of multiplex panels from commercial sources as well as custom assay development. Pathogen Detection Core Laboratory offers antibody, antigen, virus, DNA, RNA and other diagnostic marker detection for a number of infectious agents including HIV, HVP2 (Herpes B surrogate marker), LCV, RRV, SFV, SIV, SRV, STLV, SV40, TB, RhCMV and other non human primate pathogens. Services are provided to investigators and to the CNPRC colony. Custom testing, training, and consultation are also available. Respiratory Disease Immunology Core Laboratory provides technical expertise and resources to investigators interested in the immunological evaluation of rhesus macaque models for lung disease. Services include assistance with experimental design, allergen characterization and preparation, evaluation of allergen-specific IgE and IgG, and quantitation of histamine. The Core Laboratory also provides technical support for sample processing, including bronchoalveolar lavage, lymphoid tissue, lung tissue, and blood. Flow cytometry and molecular analysis of lung and blood samples are coordinated with the Flow Cytometry Core Laboratory and the Infectious Disease Immunology Core Laboratory. Immunohistochemistry of lung samples is also offered in conjunction with the Computational Imaging Core. In addition to immunological evaluation, the Respiratory Disease Immunology Core Laboratory can provide investigators with primary cell isolates and in vitro culture of rhesus lung epithelial cells and fibroblasts. In addition to these seven Laboratories, the ARC has formal links to one Affiliated Laboratory located off-site. This Laboratory is directed by a CNPRC staff scientist, and while it provides a broad range of services to the research community (including assays on human tissue), it also provides services to nonhuman primate researchers using species-specific reagents. By linking to this Laboratory, we can preclude the need to duplicate existing services and insure good communication between all service units across campus that are offering assay services of one type or another to investigators using nonhuman primates.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。对该子项目的主要支持和子弹的主要研究者可能由其他来源（包括其他NIH来源）提供。 该子项目列出的总成本可能代表了子项目使用的估计中心基础设施的估计数量，而不是NCRR赠款向子项目或副标理人员提供的直接资金。 分析和资源核心于2008年1月建立至1）提高实验室之间所有以一种或另一种形式提供的分析服务的沟通和效率； 2）提供一个更大的组织部门，可以缓冲每个实验室开展业务数量的波动； 3）促进CNPRC提供的用户访问服务，尤其是对资源的访问。 ARC的“分析”组件包括与CNPRC的先前独立核心相关的服务功能，并且该核心的“资源”组件使CNPRC上可用的病毒量，试剂，组织和其他资源可用。 由于这些资源中的许多是由提供测定服务的相同实验室开发的，因此将两个功能，服务和资源合并为一个核心。 ARC的结构包括以下七个CNPRC实验室： 临床实验室为内部CNPRC单元以及使用我们的设施提供了临床和研究支持，以监测菌落动物的健康状况，以实现常规和实验目的 内分泌核心实验室为研究人员提供激素分析的服务以及与非人类灵长类动物进行的内分泌研究有关的咨询。 该核心实验室还根据研究人员的需要开发并验证类固醇，单胺和蛋白质激素测定。 流式细胞仪核心实验室提供了使用两种仪器的访问和服务的访问权限，即Calibur FACS Calibur，可用于标准的4色测定法，而FACS ARIA具有3个激光器，可用于多色流式细胞量学分析和用于多色活细胞分类。 实验室对标准临床实验室小组进行染色，采集和分析，但主要研究人员可以要求定制面板。 遗传学核心实验室在以下领域提供研究支持：自定义DNA提取和纯化，整个基因组扩增，SNP，STR和MTDNA分析；生物信息学分析，包括整个基因组和目标区域链接和关联分析；和候选基因评估。 传染病免疫学核心实验室提供以下服务：血液和组织加工（包括粘膜组织），针对整个SIV的抗体ELISA，用于表型的流式细胞仪和多种细胞类型的功能表征（通过流式细胞仪核心实验室），RNA / DNA分离（血液和组织）和几个靶基因的分析。 此外，可用的血浆或培养上清液中的多重微粒测定法检测抗体和多个免疫调节剂。这包括使用来自商业来源的多重面板以及自定义测定开发。 病原体检测核心实验室提供抗体，抗原，病毒，DNA，RNA和其他诊断标记物检测，可用于许多感染剂，包括HIV，HVP2（疱疹B替代标记），LCV，RRV，RRV，SFV，SIV，SIV，SIV，SRV，SRV，SRV，SV40，SV40，SV40，SV40，SV40，SV40，SV40，SV40，SV40，SV40，SV40，SV40，SV40，，SV40，SV40，SV40，SV40，SV40，SV40，，SV40，SRV，SRV，SRV，SRV，SRV，SRV，SRV，SRV，SFV TB，RHCMV和其他非人类灵长类病原体。 提供服务给调查人员和CNPRC殖民地。 还提供自定义测试，培训和咨询。 呼吸道疾病免疫学核心实验室为对恒河猴猕猴模型的免疫学评估感兴趣的研究人员提供技术专长和资源。 服务包括实验设计的援助，过敏原表征和制备，过敏原特异性IgE和IgG的评估以及组胺的定量。核心实验室还为样品加工提供了技术支持，包括支气管肺泡灌洗，淋巴组织，肺组织和血液。肺和血液样本的流式细胞仪和分子分析与流式细胞仪核心实验室和传染病免疫学核心实验室协调。 肺样品的免疫组织化学也与计算成像核心一起提供。 除了免疫学评估外，呼吸道疾病免疫学核心实验室还可以为研究人员提供原代细胞分离株和恒河肺上皮细胞和成纤维细胞的体外培养。 除了这七个实验室外，ARC还与位于现场的一个附属实验室有正式的联系。 该实验室由CNPRC的员工科学家执导，尽管它为研究界（包括人体组织的测定法）提供了广泛的服务，但它还使用物种特异性试剂为非人类灵长类动物研究人员提供服务。 通过链接到该实验室，我们可以排除需要复制现有服务并确保校园内所有服务单位之间的良好沟通，这些单位正在向使用非人类灵长类动物提供一种或另一种类型的测定服务。