GEN-RED

GEN-RED

• 批准号: 7951430
• 负责人: WILLIAM Bradford LAWSON
• 金额: $ 2.13万
• 依托单位: HOWARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7951430
• 关键词: 15q; 17p; Affect; African American; Blinded; Blood specimen; Cell Culture Techniques; Cell Line; Chromosomes; Clinical; Clinical Data; Clinical Research; Collection; Computer Retrieval of Information on Scientific Projects Database; Deposition; Diagnostic; European; Family; Funding; Genome Scan; Grant; Institution; Major Depressive Disorder; Maps; Modeling; National Institute of Mental Health; Parents; Recurrence; Relative (related person); Research; Research Personnel; Resources; Sampling; Siblings; Site; Source; Susceptibility Gene; United States National Institutes of Health; Universities; base; depression; early onset; genome-wide; male; meetings; positional cloning; proband; repository; sex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a second revision of a collaborative R01 four-year competing continuation proposal to create a large repository-based sample of cases with recurrent, early-onset major depressive disorder (MDD-RE), and to use positional cloning to identify depression susceptibility genes in regions of significant linkage in our genome scan. The completed four-year project collected 680 families containing 927 affected sibling pairs (ASPs) (MDD-RE diagnostic model) and additional affected relatives (GenRED I). Blinded clinical data and blood specimens for cell culture were deposited in the NIMH repository and are being made public. Linkage fine-mapping has demonstrated genome-wide significant linkage on chromosome 15q; in the 10 cM genome scan, suggestive sex-specific linkage was observed in three regions (6p-q, 8p, 17p), with the result on chromosome 17p approaching genome-wide significance. Six collaborating sites now propose to: (1) Collect (during Years 1-3) an additional 1,350 European-ancestry (EUR) MDD-RE probands (GenRED II) meeting identical criteria (including evidence of having an affected sibling) to create a total repository sample of 2,000 EUR MDD-RE cases, plus cell lines/DMA from available parents, unaffected sibs and male-male ASPs. (2) Initiate a repository-based collection of African-American (AA) MDD-RE probands meeting the same clinical criteria.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这是 R01 四年竞争性延续提案的第二次修订，旨在创建一个基于存储库的大型复发性早发性重度抑郁症 (MDD-RE) 病例样本，并使用定位克隆来识别抑郁症易感基因在我们的基因组扫描中具有重要联系的区域。已完成的四年项目收集了 680 个家庭，其中包括 927 对受影响的兄弟姐妹 (ASP)（MDD-RE 诊断模型）和其他受影响的亲属 (GenRED I)。盲法临床数据和细胞培养的血液样本已存放在 NIMH 存储库中并正在公开。连锁精细作图已证明染色体 15q 上全基因组的显着连锁；在 10 cM 基因组扫描中，在三个区域（6p-q、8p、17p）观察到暗示性的性别特异性连锁，染色体 17p 上的结果接近全基因组显着性。六个合作中心现提议：(1)（在第 1-3 年期间）收集另外 1,350 名符合相同标准（包括有受影响兄弟姐妹的证据）的欧洲血统 (EUR) MDD-RE 先证者 (GenRED II)，以创建一个2,000 欧元 MDD-RE 病例的总存储库样本，以及来自可用父母、未受影响的同胞和男性 ASP 的细胞系/DMA。 (2) 启动基于存储库的符合相同临床标准的非裔美国人 (AA) MDD-RE 先证者收集。