SBIR TOPIC 294 PHASE 1 - DEVELOPMENT OF GLYCOSYLATION - SPECIFIC RESEARCH

SBIR 主题 294 第 1 阶段 - 糖基化的发展 - 特定研究

• 批准号: 8353245
• 负责人: YIE-HWA CHANG
• 金额: $ 14.86万
• 依托单位: MEDIOMICS, LLC
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2012-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8353245
• 关键词: Antibodies; Antigens; Biological Assay; Cancer Biology; Carbohydrates; Chickens; Development; Early Diagnosis; Glycoproteins; Goals; Human; IgY; Image; Link; Malignant - descriptor; Malignant Neoplasms; Methods; Modification; Pattern; Peptides; Phase; Post-Translational Protein Processing; Protein Glycosylation; Proteins; Reagent; Research; Research Personnel; Site; Small Business Innovation Research Grant; Specificity; Staging; Technology; Testing; Time; cancer diagnosis; cancer therapy; cancer type; glycosylation; instrument; polyclonal antibody; tumor progression

Glycosylation changes are a universal feature of malignant transformation and tumor progression. Changes in expression levels of certain glycoproteins and altered glycosylation patterns have been detected in most of the major human cancer types and at different stages of tumor progression. The early detection of glycosylation changes is therefore crucial for cancer diagnosis and treatment. Currently, the methods for glycosylation analysis require expensive instruments and are time consuming. Thus, there is a need to develop new reagents and simpler methods to study carbohydrate modifications of proteins in cancer biology. Our long term goal is to develop new research reagents for basic cancer researchers in the form of antibodies against N- or O-linked carbohydrate antigens in proteins. In the Phase I proposal, we propose to generate over 32 chicken polyclonal (Task 1) and 2-3 monoclonal IgY (Task 2) ¿ against specific Oglycosylation sites. In addition, we will use our proprietary PINCER¿ platform technology to develop 5 costeffective, simple and fast assays (Task 3) to detect protein glycosylation using existing commercially available antibodies. We expect that these PINCER¿ reagents can be used to not only develop homogeneous assays for detecting protein glycosylation, but can also be used as unique imaging reagents.

糖基化变化是恶性转化和肿瘤进展的普遍特征。更改 在某些糖蛋白的表达水平和糖基化模式的改变中 人类癌的主要类型和肿瘤进展的不同阶段。早期发现 因此，糖基化变化对于癌症诊断和治疗至关重要。目前，用于 糖基化分析需要昂贵的仪器，并且耗时。那是有必要的 开发新的试剂和更简单的方法来研究癌症蛋白质的碳氢化物修饰 生物学。我们的长期目标是以基本癌症研究人员的形式开发新的研究试剂 针对蛋白质中N-或O连接的碳水化合物抗原的抗体。在第一阶段的建议中，我们提出 产生超过32个鸡多克隆（任务1）和2-3个单克隆IGY（任务2）�针对特定的oglycosylation 站点。此外，我们将使用我们的专有夹具平台技术来开发5个成本效益， 简单而快速的测定（任务3）使用现有的商业上检测蛋白质糖基化 可用的抗体。我们期望这些钳子不仅可以用来开发 用于检测蛋白质糖基化的均匀测定法，但也可以用作独特的成像试剂。