TAS::75 0850::TAS RAPID SCREENING OF INDIVIDUAL CELLS USING SINGLE CELL MASS SPE

TAS::75 0850::TAS 使用单细胞群 SPE 快速筛选单个细胞

• 批准号: 8353018
• 负责人: THOMAS DARLINGTON
• 金额: $ 100万
• 依托单位: NANOCOMPOSIX, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-30 至 2013-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8353018
• 关键词: Aerosols; Antibodies; Cell Line; Cell Separation; Cells; Collection; Coupled; Coupling; Data; Databases; Electronics; Event; Flow Cytometry; Fluorescent Antibody Technique; Growth; Harvest; Individual; Ions; Label; Lanthanoid Series Elements; Lead; Malignant Neoplasms; Mammalian Cell; Mass Spectrum Analysis; Measures; Methods; Molecular; Phenotype; Population; Population Heterogeneity; Screening procedure; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staging; Surface; System; Techniques; Testing; Time; aerosolized; base; cancer cell; cancer diagnosis; cell type; design; detector; improved; instrument; interest; mass spectrometer; nanoparticle; operation; particle; receptor

A Single Cell time-of-flight Mass Spectrometer (SCMS) that is enabled by the use of a nanoparticle based MALDI matrix will be fabricated and tested. Unlike traditional surface-receptor-based cell phenotyping and identification techniques, the SCMS system will be able to differentiate between cell types that are indistinguishable using traditional antibody based methods. The label-free method will provide molecular information on individual cells that will lead to a new understanding of the molecular variance in heterogeneous cell mixtures. Another mode of operation allows for the use of either fluorescent, nanoparticle or elemental tags (e.g. lanthanides), to provide a highly multi-plexed labeling method using the single cell mass spectrometer as the detector. The SCMS will also have the capability to be coupled to a cytometer which can be used to isolate cell populations of interest prior to mass spectrometry analysis. Here, cell populations labeled with a fluorescent antibody can be further analyzed to measure the variance and identify sub-populations within the tagged cells.

将制造并测试使用基于纳米粒子的 MALDI 基质启用的单细胞飞行时间质谱仪 (SCMS)。与传统的基于表面受体的细胞表型分析和识别技术不同，SCMS 系统将能够区分使用传统的基于抗体的方法无法区分的细胞类型。无标记方法将提供单个细胞的分子信息，这将导致对异质细胞混合物中分子差异的新理解。另一种操作模式允许使用荧光、纳米颗粒或元素标签（例如镧系元素），以提供使用单细胞质谱仪作为检测器的高度多重标记方法。 SCMS 还能够与细胞仪连接，用于在质谱分析之前分离感兴趣的细胞群。在这里，可以进一步分析用荧光抗体标记的细胞群，以测量方差并识别标记细胞内的亚群。