Genetics of Cell Cycle & DNA Damage Regulation in Yeast

细胞周期遗传学

基本信息

批准号：
7847413
负责人：
STEPHEN J ELLEDGE
金额：
$ 34.65万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-07-01 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7847413
关键词：
Affinity Affinity Chromatography Alleles Amino Acids Anaphase Antibodies Binding Biochemical C-terminal CHEK2 gene Cations Cell Cycle Cell Cycle Arrest Cell Cycle Regulation Cells Characteristics Charge Chromatin Chromosome Segregation Chromosomes Complex Consensus Cyclic AMP-Dependent Protein Kinases Cyclin-Dependent Kinases DNA Damage DNA Double Strand Break DNA Repair DNA Replication Damage DNA biosynthesis DNA damage checkpoint Databases Daughter Defect Ensure Essential Genes Eukaryota Event Failure Future Genetic Genetic Epistasis Genetic Screening Goals Grant Homologous Gene In Vitro Investigation Ionizing radiation Iron Kinetochores Lead Left Light Cell Link MCM Protein Mammals Mass Spectrum Analysis Methods Microtubules Mitosis Mitotic Mitotic spindle Modeling Mutagenesis Mutagens Mutate Mutation N-terminal Nocodazole Nuclear Nuclear Protein Nuclear Proteins PDE2 phosphodiesterase Paint Paper Pathway interactions Peptide Fragments Peptides Phase Phenotype Phosphopeptides Phosphorylation Phosphorylation Site Phosphotransferases Play Process Protein Binding Protein Kinase Proteins Proteomics RNA Splicing Reagent Recruitment Activity Regulation Relative (related person)Resistance Role Saccharomyces cerevisiae Series Serine Set protein Signal Transduction Signal Transduction Pathway Sister Chromatid Site Stable Isotope Labeling Stress Structure Temperature Tern Trypsin Work Yeasts arginyllysine base cell cycle genetics crosslink daughter cell dosage graduate student helicase hydroxyurea in vivo inorganic phosphate interest mutant novel numb protein prevent protein function ras-Related G-Proteins repaired research study response sensor spindle pole body yeast protein

项目摘要

Our lab has been focused on how cells sense and respond to DNA damage. We have been very active n elucidating the structures sensed by the DNA damage response sensors. Through work in both yeast and mammals we have established an outline of the signal transduction pathway that is activated in response to DNA damage. This pathway consists of a protein kinase cascade. We have been very interested in understanding how the DNA damage response controls various aspects of cell cycle regulation and have therefore studied the cell cycle as well. In order to understand how the DNA damage response accomplishes its goals, it is imperative we identify the substrates of the kinases activated in response to DNA damage and elucidate the function of these substrates.

我们的实验室一直专注于细胞如何感知和响应 DNA 损伤。我们一直非常活跃 n 阐明 DNA 损伤反应传感器感知的结构。通过酵母和我们已经建立了哺乳动物信号转导途径的轮廓，该途径响应于 DNA 损伤。该途径由蛋白激酶级联组成。我们一直非常感兴趣了解 DNA 损伤反应如何控制细胞周期调节的各个方面，并具有因此也研究了细胞周期。为了了解 DNA 损伤如何反应为了实现其目标，我们必须确定响应激活的激酶的底物 DNA 损伤并阐明这些底物的功能。