NHGRI/DIR Microarray Core

NHGRI/DIR 微阵列核心

• 批准号: 8565591
• 负责人: elaine ostrander
• 金额: $ 38.68万
• 依托单位: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8565591
• 关键词: Autistic Disorder; Behavioral; Bladder Squamous Cell Carcinoma; Blood; Breeding; Canis familiaris; Cells; Copy Number Polymorphism; Development; Diabetes Mellitus; Disease; Disease model; Endometrial Carcinoma; Experimental Designs; Gaucher Disease; Gene Expression; Gene Expression Profile; Genes; Genome; Genomics; Genotype; Germ Layers; Goals; Housing; Human; Immune; Institutes; Laboratories; Lung; Malignant Neoplasms; Methylation; MicroRNAs; Microarray Analysis; Mining; Modeling; Molecular Profiling; Mus; National Human Genome Research Institute; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; Neuroglia; Neurons; Non-Insulin-Dependent Diabetes Mellitus; Nuclear Pore Complex; Phenotype; Predisposition; Progeria; Protocols documentation; Publications; RNA; Research; Research Personnel; Resources; Running; Schizophrenia; Services; Sex Differentiation; Skin; Stomach; Supraoptic Vertical Ophthalmoplegia; Syndrome; Technology; Time; Transitional Cell Carcinoma; Variant; Work; Zebrafish; base; cell motility; cost effective; genome wide association study; genotyping technology; hair cell regeneration; human disease; insight; instrumentation; meetings; melanoma; new technology; pyroglyphid; sarcoma

The NHGRI Microarray Core provides resources, cost-effective and time-efficient services, advice and technical support to meet the needs of NHGRI, NINDS, and NIMH investigators related to genomics research. The services primarily focus on gene expression and genotyping technologies. Researchers utilize the expertise, protocols and instrumentation available in the Core laboratory. The Core adapts readily as new technologies emerge, and aids in both performing laboratory services as well as experimental design. In the past year gene expression services were provided using mostly commercial (Affymetrix, Agilent and Illumina) arrays. In-house arrays, however, were available for zebrafish expression studies. The Core provided expression analysis using >1600 arrays (Affymetrix, Agilent, Illumina and In-house oligos chips). The investigators utilized the expression services (total RNA and microRNAs) to gain insight into a variety of human disease conditions, including Niemann-Pick disease, type C (NPC), melanoma, endometrial cancer, Progeria syndrome, type II diabetes, blood malignancies, and Gaucher disease. We have also been involved in expression studies of immune cell characterization and normal skin bacterial flora. In addition, studies of disease models and developmental changes in the mouse have been done. Studies include mouse lung sensitivity due to to dust mites, and glial cell migration. With regard to human disease a variety of studies have been done including those aimed at understanding expression profiles associated with schizophrenia, autism and other behavioral phenotypes. Finally, zebrafish germ layer development, sex differentiation, and hair cell regeneration have also studied this year employing the gene expression technologies of the Array Core. SNP-based microarray technologies from Affymetrix and Illumina were used by the Core to provide genotyping services critical for genome wide association (GWAS) studies. These services were applied for a variety of studies related to human neuronal diseases, cancer, and diabetes. In addition, the core optimized SNP chip technologies related to the canine model, and as a result several studies were undertaken to find genes that control morphologic variation across the species, as well as breed susceptibility to various forms of cancer including gastric, histocytic sarcoma, transitional cell carcinoma of the bladder, and squamous cell carcinoma. The dog studies alone involved running over 1,500 SNP chips. In summary, the NHGRI Array Core has met the needs to multiple investigators in three Institutes for both expression and SNP-based genotyping studies. A number of publications arose from this work and Core staff have been listed as authors on nine and cited in the acknowledgements of several others.

NHGRI微阵列核心提供资源，具有成本效益和时间效率的服务，建议和技术支持，以满足NHGRI，NINDS和NIMH研究人员与基因组学研究有关的需求。这些服务主要关注基因表达和基因分型技术。研究人员利用核心实验室中提供的专业知识，协议和仪器。随着新技术的出现，核心很容易适应，并辅助执行实验室服务和实验设计。 在过去的一年中，基因表达服务是使用商业（Affymetrix，Agilent和Illumina）阵列提供的。但是，内部阵列可用于斑马鱼表达研究。 核心提供了使用> 1600个阵列（Affymetrix，Agilent，Illumina和内部寡核酸芯片）的表达分析。 研究人员利用表达服务（总RNA和microRNA）深入了解各种人类疾病状况，包括Niemann-Pick疾病，C型（NPC），黑色素瘤，子宫内膜癌，后代综合征，II型糖尿病，血液恶性肿瘤，血液恶性肿瘤和Gaucher病。 我们还参与了免疫细胞表征和正常皮肤细菌菌群的表达研究。 此外，已经进行了疾病模型和小鼠发育变化的研究。 研究包括由于粉尘螨引起的小鼠肺敏感性以及神经胶质细胞迁移。 关于人类疾病，已经进行了各种研究，其中包括旨在了解与精神分裂症，自闭症和其他行为表型相关的表达谱的研究。 最后，斑马鱼生殖层的发育，性别分化和毛细胞再生也使用阵列核心的基因表达技术研究了。 核心使用了来自Affymetrix和Illumina的基于SNP的微阵列技术，以提供对基因组广泛关联（GWAS）研究至关重要的基因分型服务。这些服务用于与人类神经元疾病，癌症和糖尿病有关的各种研究。 此外，核心优化的SNP芯片技术与犬模型有关，结果进行了一些研究，以找到控制整个物种之间形态变异的基因，以及对各种形式的癌症的敏感性，包括胃细胞肉瘤，包括胃细胞肉瘤，过渡性细胞癌的过渡性细胞癌，膀胱和狗癌。 仅狗的研究就涉及超过1,500个SNP芯片。 总而言之，NHGRI阵列核心满足了三个研究机构的多个研究者的需求，以进行基于表达和基于SNP的基因分型研究。 这项工作出现了许多出版物，核心人员已被列为九名作者，并在其他一些人的致谢中引用。