Detection of Prions

朊病毒检测

• 批准号: 8336296
• 负责人: BYRON CAUGHEY
• 金额: $ 97.61万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8336296
• 关键词: Agriculture; Biological; Biological Assay; Bovine Spongiform Encephalopathy; Cerebrospinal Fluid; Chronic Wasting Disease; Clinic; Clinical; Creutzfeldt-Jakob Syndrome; Data; Detection; Diagnosis; Diagnostic tests; Disease; Hamsters; Human; Infection; Liquid substance; Mus; Neurodegenerative Disorders; Plasma; Prion Diseases; Prions; Protein Isoforms; Sampling; Scrapie; Sensitivity and Specificity; Sheep; Testing; Training; base; inhibitor/antagonist; polymerization; pre-clinical; prion seeds

The pathological isoform of the prion protein (PrPres) can serve as a marker for prion diseases, but more practical tests are needed for preclinical diagnosis and sensitive detection of many prion infections. During FY2011, we 1) developed a new prion assay (called eQuIC) that allows for the ultrasensitive and rapid of prions in inhibitor-laden biological fluids such as blood plasma; 2) adapted eQuIC to the detection of human, sheep, hamster and mouse prions in blood plasma samples; 3) trained and collaborated with human TSE surveillance groups to evaluate the utility of our previously developed RT-QuIC assay for the diagnosis of human sporadic Creutzfeldt-Jakob disease; the data showed that when applied to clinical cerebral spinal fluid samples, RT-QuIC has better sensitivity and specificity than any prior diagnostic test.

prion蛋白（PRPRES）的病理同工型可以作为prion疾病的标志物，但是临床前诊断和对许多prion感染的敏感检测需要更多的实际检测。在2011财年期间，我们1）开发了一种新的prion测定法（称为等分），该测定法可以在抑制剂含有抑制剂的生物流体（如血浆）中超敏和快速； 2）适应血浆样品中人类，绵羊，仓鼠和小鼠王子的适应性； 3）与人类TSE监视组进行了培训和合作，以评估我们先前开发的RT Quic分析的实用性，以诊断人类零星的Creutzfeldt-Jakob病；数据表明，当应用于临床脑脊柱样品时，RT Quic具有比任何先前的诊断测试更好的灵敏度和特异性。