Structures and Activities of Prions and Prion Proteins

朊病毒和朊病毒蛋白的结构和活性

• 批准号: 8336077
• 负责人: BYRON CAUGHEY
• 金额: $ 72.15万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8336077
• 关键词: Bovine Spongiform Encephalopathy; Chronic Wasting Disease; Creutzfeldt-Jakob Syndrome; Deuterium; Disease; Goals; Hydrogen; Molecular; Molecular Conformation; Mus; NMR Spectroscopy; Neurodegenerative Disorders; Pathogenesis; Prion Diseases; Prions; Recombinants; Relative (related person); Scrapie; Spectrum Analysis; Structure; beta pleated sheet; disease transmission; glycosylation; infrared spectroscopy; prion seeds; solid state nuclear magnetic resonance; transmission process

Accomplishments for FY2011: 1) We have studied the structures of prion seeded recombinant PrP fibrils by infrared spectroscopy and solid-state nuclear magnetic resonance spectroscopy and found evidence for parallel-inregister beta sheet conformations in portions of those fibrils. 2) Using a combination of infrared and hydrogen-deuterium exchanges spectroscopies we have discriminated strain-dependent conformations of mouse scrapie prion protein and determined that the effects of glycosylation and GPI-anchoring on the conformations are modest relative to the conformational differences between strains.

2011财年的成就：1）我们通过红外光谱和固态核磁共振光谱研究了Prion播种重组PRP原纤维的结构，并找到了这些原纤维部分中并行内注射β构象构象的证据。 2）使用红外和氢 - 脱水液交换的组合，我们具有鉴于小鼠抓蛋白蛋白质的应变依赖性构象，并确定相对于菌株之间构象的构象而言，糖基化和gpi粘结对构象的影响是适度的。