STEM CELL PRODUCTION CORE

干细胞生产核心

• 批准号: 8358074
• 负责人: Bruce A. Bunnell
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358074
• 关键词: Adipocytes; Adipose tissue; Adult; Age; Animals; Biomedical Research; Bone Marrow; Cell Line; Cells; Chondrocytes; Communities; Core Facility; Disease; Funding; Generations; Gestational Age; Grant; Health Sciences; Hereditary Disease; Inflammatory Response; Macaca mulatta; Maintenance; Marrow; Medical; Mesenchymal Stem Cells; Muscle Cells; National Center for Research Resources; Newborn Infant; Osteoblasts; Primates; Principal Investigator; Process; Production; Property; Research; Research Infrastructure; Research Project Grants; Resources; Scientist; Source; Stem cells; Stromal Cells; Techniques; Therapeutic; Tissue Sample; United States National Institutes of Health; adult stem cell; cell bank; cell preparation; cell type; cost; interest; nonhuman primate; sex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Mesenchymal stem cells or marrow stromal cells (MSCs) are a subset of adult stem cells from bone marrow. These cells are of medical and therapeutic interest because they have been shown to differentiate into osteoblasts, adipocytes, chondrocytes and myocytes. In addition these cell types have been shown to squelch inflammatory response associated with disease processes. Due to their biologic properties, these cells have the potential to be useful for the treatment of a large number of genetic diseases. Dr. Bunnell has successfully defined the requirements for the expansion and characterization of rhesus macaque MSCs isolated from either the bone marrow or adipose tissue. The Stem Cell Production Core Facility (SCPC) focuses on generation, maintenance and distribution of nonhuman primate MSCs. MSCs are regularly isolated from rhesus macaque bone marrow and adipose tissue samples. We presently have a bank of MSC cell lines generated from over 100 rhesus macaques of varying age prepared and ready for distribution. The SCPC presently has a large impact on other divisions within the TNPRC, and Departments and Centers within the Tulane Health Sciences Center and the Pennington Biomedical Research Center and other research labs nationally. This core produces rhesus macaque stem cell preparations for collaborative research projects. For this we plan to use the techniques developed over the last funding period to isolate stem cells (MSCs and ESCs) to expand the bank of various stem cell types isolated from animals of various ages (fetal, newborn and adults) and sexes of animals. The cells will be used to generate large cell banks for use internally or externally by collaborating scientists. We have generated an extensive bank of stem cells for use by the scientific community for various ages of animals.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 间充质干细胞或骨髓基质细胞（MSC）是来自骨髓的成体干细胞的一个子集。这些细胞具有医学和治疗意义，因为它们已被证明可以分化为成骨细胞、脂肪细胞、软骨细胞和肌细胞。此外，这些细胞类型已被证明可以抑制与疾病过程相关的炎症反应。由于其生物学特性，这些细胞有可能用于治疗大量遗传疾病。 Bunnell 博士成功地确定了从骨髓或脂肪组织中分离的恒河猴 MSC 的扩增和表征的要求。 干细胞生产核心设施 (SCPC) 专注于非人类灵长类 MSC 的生成、维护和分配。定期从恒河猴骨髓和脂肪组织样本中分离出 MSC。我们目前拥有由 100 多只不同年龄的恒河猴生成的 MSC 细胞系库，已准备好并准备分发。 SCPC 目前对 TNPRC 内的其他部门、杜兰健康科学中心和彭宁顿生物医学研究中心以及全国其他研究实验室内的部门和中心有很大影响。 该核心为合作研究项目生产恒河猴干细胞制剂。为此，我们计划使用上一个资助期间开发的技术来分离干细胞（MSC 和 ESC），以扩大从不同年龄（胎儿、新生儿和成人）和动物性别的动物中分离出的各种干细胞类型的库。这些细胞将用于生成大型细胞库，供合作科学家在内部或外部使用。我们已经建立了一个广泛的干细胞库，供科学界用于不同年龄的动物。