STEM CELL PRODUCTION CORE

干细胞生产核心

• 批准号: 8358074
• 负责人: Bruce A. Bunnell
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358074
• 关键词: Adipocytes; Adipose tissue; Adult; Age; Animals; Biomedical Research; Bone Marrow; Cell Line; Cells; Chondrocytes; Communities; Core Facility; Disease; Funding; Generations; Gestational Age; Grant; Health Sciences; Hereditary Disease; Inflammatory Response; Macaca mulatta; Maintenance; Marrow; Medical; Mesenchymal Stem Cells; Muscle Cells; National Center for Research Resources; Newborn Infant; Osteoblasts; Primates; Principal Investigator; Process; Production; Property; Research; Research Infrastructure; Research Project Grants; Resources; Scientist; Source; Stem cells; Stromal Cells; Techniques; Therapeutic; Tissue Sample; United States National Institutes of Health; adult stem cell; cell bank; cell preparation; cell type; cost; interest; nonhuman primate; sex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Mesenchymal stem cells or marrow stromal cells (MSCs) are a subset of adult stem cells from bone marrow. These cells are of medical and therapeutic interest because they have been shown to differentiate into osteoblasts, adipocytes, chondrocytes and myocytes. In addition these cell types have been shown to squelch inflammatory response associated with disease processes. Due to their biologic properties, these cells have the potential to be useful for the treatment of a large number of genetic diseases. Dr. Bunnell has successfully defined the requirements for the expansion and characterization of rhesus macaque MSCs isolated from either the bone marrow or adipose tissue. The Stem Cell Production Core Facility (SCPC) focuses on generation, maintenance and distribution of nonhuman primate MSCs. MSCs are regularly isolated from rhesus macaque bone marrow and adipose tissue samples. We presently have a bank of MSC cell lines generated from over 100 rhesus macaques of varying age prepared and ready for distribution. The SCPC presently has a large impact on other divisions within the TNPRC, and Departments and Centers within the Tulane Health Sciences Center and the Pennington Biomedical Research Center and other research labs nationally. This core produces rhesus macaque stem cell preparations for collaborative research projects. For this we plan to use the techniques developed over the last funding period to isolate stem cells (MSCs and ESCs) to expand the bank of various stem cell types isolated from animals of various ages (fetal, newborn and adults) and sexes of animals. The cells will be used to generate large cell banks for use internally or externally by collaborating scientists. We have generated an extensive bank of stem cells for use by the scientific community for various ages of animals.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 间充质干细胞或骨髓基质细胞（MSC）是骨髓中成年干细胞的子集。这些细胞具有医学和治疗兴趣，因为它们已被证明将其分化为成骨细胞，脂肪细胞，软骨细胞和心肌细胞。另外，这些细胞类型已显示出与疾病过程相关的炎症反应。由于其生物学特性，这些细胞具有可用于治疗大量遗传疾病的潜力。 Bunnell博士成功地定义了从骨髓或脂肪组织中分离出的恒河猕猴MSC的需求。 干细胞生产核心设施（SCPC）着重于非人类灵长类动物MSC的生成，维护和分布。 MSC经常从猕猴骨髓和脂肪组织样品中分离出来。目前，我们有一组MSC细胞系，由100多个恒河猕猴生成，准备了不同的年龄并准备好分发。 SCPC目前对TNPRC内部的其他部门以及杜兰健康科学中心以及彭宁顿生物医学研究中心和其他全国其他研究实验室的部门和中心产生了巨大影响。 该核心为协作研究项目生成恒河猕猴的干细胞准备。为此，我们计划使用在最后一个资金期间开发的技术分离干细胞（MSC和ESC），以扩大从各个年龄（胎儿，新生儿和成人）和动物性别的动物中分离出的各种干细胞类型的库。这些细胞将用于生成大型细胞库，以通过协作科学家在内部或外部使用。我们已经生成了大量的干细胞，供科学界用于各个年龄的动物。