STEM CELL PRODUCTION CORE: ADULT ANIMAL MARROW STEM CELLS

干细胞生产核心：成年动物骨髓干细胞

• 批准号: 8172969
• 负责人: Bruce A. Bunnell
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172969
• 关键词: Adipocytes; Adipose tissue; Adult; Age; Alcohols; Animals; Astrocytes; Biological Assay; Biomedical Research; Bone Marrow; Cell Line; Cells; Chondrocytes; Chronic; Communities; Comparative Pathology; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Development; Event; Frequencies; Funding; Generations; Gestational Age; Globoid cell leukodystrophy; Goals; Grant; Health Sciences; Hereditary Disease; In Vitro; Institution; Macaca mulatta; Maintenance; Marrow; Mediating; Medical; Mesenchymal Stem Cells; Molecular; Muscle Cells; Neurons; Newborn Infant; Oligodendroglia; Osteoblasts; Production; Property; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; SIV; Scientist; Source; Stem cells; Stromal Cells; Techniques; Therapeutic; Tissue Sample; United States National Institutes of Health; adult stem cell; cell bank; cell preparation; cell type; gene therapy; interest; mature animal; nonhuman primate; programs; relating to nervous system; sex; stem cell population

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mesenchymal stem cells or marrow stromal cells (MSCs) are a subset of adult stem cells from bone marrow. These cells are of medical and therapeutic interest because they have been shown to differentiate into osteoblasts, adipocytes, chondrocytes, myocytes, astrocytes, oligodendrocytes and neurons. Due to their inherent plasticity, these cells have the potential to be useful for the treatment of a large number of genetic diseases. Dr. Bunnell has successfully defined the requirements for the expansion and characterization of rhesus macaque MSCs. The Stem Cell Production Core Facility (SCPC) focuses on generation, maintenance and distribution of nonhuman primate MSCs. We routinely prepare MSCs from rhesus macaque bone marrow and adipose tissue samples. We presently have a bank pf MSC cell lines generated from over 100 rhesus macaques of varying age prepared and ready for distribution. The SCPC presently has a large impact not only on the Gene Therapy Program, but on other divisions such as Comparative Pathology within the TNPRC, but also Departments and Centers within the Tulane Health Sciences Center and the Pennington Biomedical Research Center and other research labs nationally. Produce rhesus macaque stem cell preparations for collaborative research projects. For this we plan to use the techniques developed over the last funding period to isolate stem cells (MSCs, NSPCs and ESCs) to expand the bank of various stem cell types isolated from animals of various ages (fetal, newborn and adults) and sexes of animals. The cells will be used to generate large cell banks for use internally or externally by collaborating scientists. We have generated an extensive bank of stem cells for use by the scientific community for various ages of animals. Continue the characterization of nonhuman primate stem cells. Our goal in these studies will be to delineate the cellular and molecular events that take place during maturation and/or differentiation of the various stem cell populations. Scientists in the division will continue to compare the biologic properties of the various stem cells in vitro. Assays mediating in vitro differentiation specific to rhesus stem cells that will maximize the differentiation and proliferation of MSCs and their progeny will be generated. One focus of the in vitro characterization will be the differentiation along neural lineages for all of the stem cells described. In order to accomplish the treatment of Krabbe's disease, stem cells will need to undergo differentiation into oligodendrocytes. A significant effort will be made towards the development and application of differentiation protocols to accomplish this goal. Over the past year, we have collaborated with investigators form LSU on the characterization of the frequency and biologic properties of MSCs isolated form rhesus macaques exposed to chronic alcohol and infected with SIV to assess the effects on the composition bone marrow cavity and stem cells.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 间充质干细胞或骨髓基质细胞（MSC）是骨髓中成年干细胞的子集。这些细胞具有医学和治疗性的兴趣，因为它们已被证明分化为成骨细胞，脂肪细胞，软骨细胞，肌细胞，星形胶质细胞，少突胶质细胞和神经元。由于其固有的可塑性，这些细胞有可能有助于治疗大量遗传疾病。 Bunnell博士成功地定义了猕猴MSC的扩展和表征的要求。 干细胞生产核心设施（SCPC）着重于非人类灵长类动物MSC的生成，维护和分布。我们通常从恒河猴骨髓和脂肪组织样品中制备MSC。目前，我们有一个由100多个恒河猕猴生成的Bank PF MSC细胞系，准备了不同的年龄并准备好分发。 SCPC目前不仅对基因治疗计划产生了很大的影响，而且对TNPRC内的比较病理等其他部门产生了巨大影响，而且对Tulane Health Sciences Center和Pennington Biomedical研究中心和全国其他研究实验室的部门和中心也产生了巨大影响。 为合作研究项目生产恒河猴干细胞准备。为此，我们计划使用在上一个资金期间开发的技术来分离干细胞（MSC，NSPC和ESC），以扩大从各个年龄（胎儿，新生儿和成人的动物）和性别分离的各种干细胞类型的库以及动物。这些细胞将用于生成大型细胞库，以通过协作科学家在内部或外部使用。我们已经生成了大量的干细胞，供科学界用于各个年龄的动物。 继续表征非人类灵长类动物干细胞。在这些研究中，我们的目标是描述各种干细胞种群成熟和/或分化过程中发生的细胞和分子事件。该部门的科学家将继续比较体外各种干细胞的生物学特性。将在最大化MSC及其后代的分化和增殖的恒河猴特异性分化的体外分化的测定中。体外表征的一个焦点将是所有描述的干细胞沿神经谱系的分化。为了完成克拉布氏病的治疗，干细胞将需要分化为少突胶质细胞。为了实现这一目标，将为开发和应用分化协议做出巨大的努力。在过去的一年中，我们与研究人员合作了LSU，以表征MSC的频率和生物学特性，分离出暴露于慢性酒精并感染SIV以评估对组成骨髓腔和干细胞的影响。