OVARIAN TISSUE CRYOPRESERVATION IN NONHUMAN PRIMATES

非人类灵长类动物的卵巢组织冷冻保存

• 批准号: 8357823
• 负责人: Mary B Zelinski
• 金额: $ 5.82万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357823
• 关键词: 3-Dimensional; Autologous; Biocompatible Materials; Biological Preservation; Cancer Patient; Cancer Survivor; Clinical; Cryopreservation; Embryonic Development; Encapsulated; Female; Fertility; Freezing; Funding; Goals; Grant; Human; Leadership; Life; Macaca; Malignant Neoplasms; Methods; Morphology; National Center for Research Resources; Oocytes; Ovarian; Ovarian Tissue; Patients; Physicians; Polymers; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Risk; Solutions; Source; Technology; Time; Tissues; Transplantation; United States National Institutes of Health; Universities; Woman; cost; improved; nonhuman primate; novel; offspring; steroid hormone

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project is part of a Roadmap Consortium (U54 RR02437, T. Woodruff, PI) entitled "The Oncofertility Consortium: Fertility Preservation for Women". The goal is to provide leadership in state-of-the-art cryopreservation technology to practitioners who are preserving human ovarian tissues under the aegis of the National Physicians Cooperative. The specific aim is to optimize ovarian cryopreservation methods in nonhuman primates for restoring fertility in female cancer survivors by comparing novel vitrification freeze-thaw methods to the currently utilized slow freeze method. Although ovarian transplantation after cryopreservation has yielded live human offspring, autologous transfer in many cancer patients carries the risk of re-seeding the patient with her own cancer. In these patients, encapsulated 3-dimensional (3D) culture of isolated secondary follicles post-thaw may be the best option for fertility preservation. Since slow freezing damages secondary follicles, optimization of ovarian tissue vitrification could bridge a technical gap for patients to utilize frozen ovarian tissue outside of transplantation. For the first time, macaque follicles isolated from vitrified tissue can survive, grow, form an antrum and produce steroid hormones, indicating functional preservation of cryopreserved secondary follicles. The addition of non-permeating polymers to the vitrification solution improved tissue morphology and follicle function in 3D culture. Current efforts are aimed at producing a mature oocyte derived from 3D follicle culture that can fertilize, undergo embryonic development and ultimately produce a live offspring. This project is facilitated by interactions with the Biomaterials Core at Northwestern University. Expected advances will be transferred to clinical efforts at Fertility Centers.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目是路线图联盟（U54 RR02437，T。Woodruff，PI）的一部分，名为“妇产金协会：妇女的生育能力保存”。目的是为在国家医生合作的宙斯盾下保存人类卵巢组织的从业人员提供领导力。 具体的目的是通过将新型的玻璃化冻结方法与当前使用的慢冻结方法进行比较，以优化非人类灵长类动物的卵巢冷冻​​保存方法来恢复女性癌症幸存者的生育能力。 尽管冷冻保存后的卵巢移植产生了人类的后代，但许多癌症患者的自体转移却有重新种植患者自己癌症的风险。在这些患者中，分离后毛囊的封装3维（3D）培养物可能是保存生育能力的最佳选择。 由于较慢的冻结损害次级卵泡，因此优化卵巢组织玻璃化可能会弥合技术差距，以便患者利用移植以外的冷冻卵巢组织。从玻璃体组织中分离出来的猕猴可以生存，生长，形成鼻窦并产生类固醇激素，表明冷冻保存的次级卵泡的功能保存。在玻璃化溶液中添加非渗透聚合物改善了3D培养中的组织形态和卵泡功能。 当前的努力旨在产生来自3D卵泡培养物的成熟卵母细胞，该卵泡培养物可以施肥，经历胚胎发育并最终产生活的后代。 与西北大学的生物材料核心相互作用，该项目促进了该项目。 预期的进步将转移到生育中心的临床工作中。