DEVELOPMENT OF A PRIMATE GENOMICS RESOURCE

灵长类基因组资源的开发

• 批准号: 8357630
• 负责人: MICHAEL G KATZE
• 金额: $ 37.79万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357630
• 关键词: Acquired Immunodeficiency Syndrome; Acute; Animal Experimentation; Animals; Communities; Complement; Databases; Development; Event; Funding; Genomics; Goals; Grant; Immune response; Individual; Infection; Inflammatory; Internet; Intervention; Macaca; Macaca mulatta; Mass Spectrum Analysis; Modality; Modeling; National Center for Research Resources; Natural Immunity; Outcome; Pathway interactions; Primates; Principal Investigator; Process; Proteins; Proteomics; Protocols documentation; Research; Research Infrastructure; Research Personnel; Resolution; Resources; SIV; Source; System; Systems Biology; Technology; Tissues; United States National Institutes of Health; Vaccines; Virus Diseases; cost; functional genomics; male; nonhuman primate; protein profiling; rectal; response; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary goal of the model remains the same as in the original protocol. This project is focused on creating genomic/proteomic resources that can be applied to research using nonhuman primates. Through the construction of proteomic resources, we intend to provide the research community with the full complement of species-specific genomic and proteomic tools needed to apply the technologies of functional genomics to nonhuman primate research. We will use sensitive, high-resolution mass spectrometry to build databases of identified proteins present in various rhesus macaque (Macaca mulatta) tissues. These databases will be used to characterize the changes in cellular protein profiles that occur in response to simian immunodeficiency virus infection. These studies will allow us to evaluate these resources in an experimental system that will enhance the use of macaques as models for AIDS research. The results will be shared via Web portals, as a resource to enable researchers to maximize the types and amounts of information that can be obtained from these valuable research animals. We will perform a systems biology interrogation of an acute mucosal infection model, employing intra-rectal challenge with SIVmac251 in 24 male, Indian-origin rhesus macaques. The primary hypothesis is that a detailed systems level analysis of early changes in the acute infection with a pathogenic strain of SIV will reveal events in innate immunity, inflammatory processes, and the ensuing transition to the adaptive immune response. As one corollary to this hypothesis, these features may serve as prognosticators of outcome, as reflected in the variability of individual animals. A second corollary is that such a systems level analysis will identify specific targets and pathways for intervention by pharmacologic agents or vaccine modalities

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该模型的主要目标与原始协议中相同。该项目的重点是创建基因组/蛋白质组学资源，这些资源可用于使用非人类灵长类动物进行研究。通过构建蛋白质组学资源，我们打算为研究界提供全面的物种特异性基因组和蛋白质组学工具，将功能基因组学技术应用于非人类灵长类动物研究。我们将使用敏感的高分辨率质谱法来构建各种恒河猕猴（Macaca Mulatta）组织中存在的蛋白质的数据库。这些数据库将用于表征响应于猿猴免疫缺陷病毒感染而发生的细胞蛋白谱的变化。这些研究将使我们能够在实验系统中评估这些资源，以增强猕猴作为艾滋病研究的模型。结果将通过Web门户网站共享，以使研究人员能够最大程度地利用这些宝贵的研究动物获得的信息类型和量。 我们将对急性粘膜感染模型进行系统生物学询问，并在24个男性印度 - 印度 - 原始恒河猕猴中对直肠内挑战采用直肠内挑战。 主要假设是，用SIV的致病菌株对急性感染的早期变化进行了详细的系统水平分析，将揭示先天免疫，炎症过程中的事件以及随之而来的向适应性免疫反应的过渡。作为这一假设的一种推论，这些特征可以用作结果的预后，如单个动物的变异性所反映。第二个推论是，这样的系统级分析将确定药物或疫苗方式干预的特定目标和途径