INTRARECTAL TITRATION OF SHIV 162P4 STOCK

SHIV 162P4 库存的直肠内滴定

• 批准号: 8357586
• 负责人: Shiu-Lok Hu
• 金额: $ 32.86万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357586
• 关键词: Animals; Antibodies; Antibody Formation; CCR5 gene; Comparative Study; Data; Epitopes; Evolution; Family suidae; Funding; Goals; Grant; HIV-1; Infection; Macaca; Macaca mulatta; Molecular Conformation; Monitor; National Center for Research Resources; Pathogenicity; Peripheral Blood Mononuclear Cell; Primates; Principal Investigator; Reporting; Research; Research Infrastructure; Resources; Route; Source; Tail; Titrations; United States National Institutes of Health; Universities; Viral; Virus Diseases; cost; in vivo; neutralizing antibody; neutralizing monoclonal antibodies; simian human immunodeficiency virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We previously reported a comparative study of the in vivo infectivity and pathogenicity of a CCR5-tropic SHIVSF162 P4 in rhesus and pig-tailed macaques. Results indicate that both rhesus and pig-tailed macaques are similarly susceptible to SHIVSF162 P4 infection by mucosal routes. However, SHIV replication was significantly more robust in pig-tailed macaques than in rhesus. Since, persistent viral infection has been correlated with broadening of neutralizing antibody responses, we continued to monitor the evolution of viral sequences and neutralizing antibody activities in a subset of infected pig-tailed macaques after the primary goal of this study was achieved. PBMC from some of these animals were sent to Dr. James Robinson at Tulane University to isolate neutralizing monoclonal antibodies against HIV-1. Preliminary data from Dr. Robinson indicate that some of these antibodies may recognize conformation-dependent epitopes against HIV-1 envelope. Further characterization of these antibodies is in progress.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们之前报道了一项 CCR5 趋向性 SHIVSF162 P4 在恒河猴和猪尾猕猴体内感染性和致病性的比较研究。结果表明，恒河猴和猪尾猕猴同样容易通过粘膜途径感染 SHIVSF162 P4。然而，猪尾猕猴中的 SHIV 复制明显强于恒河猴。由于持续性病毒感染与中和抗体反应的扩大有关，因此在实现本研究的主要目标后，我们继续监测一部分受感染猪尾猕猴中病毒序列的演变和中和抗体活性。来自其中一些动物的 PBMC 被送往杜兰大学的 James Robinson 博士处，以分离出针对 HIV-1 的中和单克隆抗体。 Robinson 博士的初步数据表明，其中一些抗体可能识别针对 HIV-1 包膜的构象依赖性表位。这些抗体的进一步表征正在进行中。