INTRARECTAL TITRATION OF SHIV 162P4 STOCK

SHIV 162P4 库存的直肠内滴定

• 批准号: 8172740
• 负责人: Shiu-Lok Hu
• 金额: $ 46.53万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172740
• 关键词: Antibody Formation; Comparative Study; Computer Retrieval of Information on Scientific Projects Database; Evolution; Family suidae; Funding; Generations; Goals; Grant; HIV-1; Infection; Institution; Macaca; Macaca mulatta; Modeling; Monitor; Pathogenicity; Reporting; Research; Research Personnel; Resources; Route; Source; Tail; Titrations; United States National Institutes of Health; Viral; Virus Diseases; in vivo; neutralizing antibody; simian human immunodeficiency virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We previously reported a comparative study of the in vivo infectivity and pathogenicity of a CCR5-tropic SHIVSF162 P4 in rhesus and pig-tailed macaques. Results indicate that both rhesus and pig-tailed macaques are similarly susceptible to SHIVSF162 P4 infection by mucosal routes. However, SHIV replication was significantly more robust in pig-tailed macaques than in rhesus. Since, persistent viral infection has been correlated with broadening of neutralizing antibody responses, we continued to monitor the evolution of viral sequences and neutralizing antibody activities in a subset of infected pig-tailed macaques after the primary goal of this study was achieved. Information obtained from these studies may help the establishment of macaque models to determine factors required for the generation of broadly neutralizing antibodies against HIV-1.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 我们此前曾报道过对恒河猴和猪尾猕猴中CCR5-循环SHIVSF162 P4的体内感染性和致病性的比较研究。结果表明，恒河猴和猪尾猕猴同样容易受到粘膜途径的Shivsf162 P4感染。然而，猪尾猕猴的湿夫复制比在恒河猴中更强大。由于，持续的病毒感染与中和中和抗体反应的扩展相关，因此在实现了这项研究的主要目标之后，我们继续监测病毒序列的演变和中和抗体活性的演变。从这些研究中获得的信息可能有助于建立猕猴模型，以确定生成针对HIV-1的广泛中和抗体所需的因素。